{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Djalali-Cuevas A"],"funding":["European Research Council","Science Foundation Ireland","Horizon 2020","H2020","University of Galway","H2020 Marie Skłodowska-Curie Actions","European Regional Development Fund"],"pagination":["100977"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10846491"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25"],"pubmed_abstract":["Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged <i>ex vivo</i> time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelerate the development of functional tissue facsimiles, herein we compared human tenocyte and dermal fibroblast behaviour under standard and macromolecular crowding conditions to inform future studies in tendon engineering. Basic cell function analysis made apparent the innocuousness of macromolecular crowding for both cell types. Gene expression analysis of the without macromolecular crowding groups revealed expression of tendon related molecules in human dermal fibroblasts and tenocytes. Pr"],"journal":["Materials today. Bio"],"pubmed_title":["Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis."],"pmcid":["PMC10846491"],"funding_grant_id":["676338","13/RC/2073_2","19/FFP/6982","866126","15/CDA/3629"],"pubmed_authors":["Savitski M","Tzora A","Stein F","Kelly J","Diakakis N","Zeugolis DI","Prassinos N","Skoufos I","Rettel M","Biggs M","Djalali-Cuevas A","Kearns S"],"additional_accession":[]},"is_claimable":false,"name":"Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis.","description":"Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged <i>ex vivo</i> time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelerate the development of functional tissue facsimiles, herein we compared human tenocyte and dermal fibroblast behaviour under standard and macromolecular crowding conditions to inform future studies in tendon engineering. Basic cell function analysis made apparent the innocuousness of macromolecular crowding for both cell types. Gene expression analysis of the without macromolecular crowding groups revealed expression of tendon related molecules in human dermal fibroblasts and tenocytes. Pr","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-03-27T16:34:47.37Z","creation":"2025-04-05T20:42:16.909Z"},"accession":"S-EPMC10846491","cross_references":{"pubmed":["38322661"],"doi":["10.1016/j.mtbio.2024.100977"]}}