biostudies-literatureBin Haidar HMedical Research CouncilRCUK | Medical Research Council3184https://www.ebi.ac.uk/biostudies/studies/S-EPMC10850160biostudies-literatureUnknown14(1)Local tissue damage following snakebite envenoming remains a poorly researched area. To develop better strategies to treat snakebites, it is critical to understand the mechanisms through which venom toxins induce envenomation effects including local tissue damage. Here, we demonstrate how the venoms of two medically important Indian snakes (Russell's viper and cobra) affect human skeletal muscle using a cultured human myoblast cell line. The data suggest that both venoms affect the viability of myoblasts. Russell's viper venom reduced the total number of cells, their migration, and the area of focal adhesions. It also suppressed myogenic differentiation and induced muscle atrophy. While cobra venom decreased the viability, it did not largely affect cell migration and focal adhesions. CobraScientific reportsDifferential effects of the venoms of Russell's viper and Indian cobra on human myoblasts.PMC10850160MR/W019353/1Senthilkumaran SAlmeida JRBigot ABin Haidar HWilliams JGuo BVaiyapuri SPatel KfalseDifferential effects of the venoms of Russell's viper and Indian cobra on human myoblasts.Local tissue damage following snakebite envenoming remains a poorly researched area. To develop better strategies to treat snakebites, it is critical to understand the mechanisms through which venom toxins induce envenomation effects including local tissue damage. Here, we demonstrate how the venoms of two medically important Indian snakes (Russell's viper and cobra) affect human skeletal muscle using a cultured human myoblast cell line. The data suggest that both venoms affect the viability of myoblasts. Russell's viper venom reduced the total number of cells, their migration, and the area of focal adhesions. It also suppressed myogenic differentiation and induced muscle atrophy. While cobra venom decreased the viability, it did not largely affect cell migration and focal adhesions. Cobra2024-01-01T00:00:00Z2024 Feb2025-04-04T10:42:06.03Z2025-04-04T10:42:06.03ZS-EPMC108501603832645010.1038/s41598-024-53366-9