{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krishnarjuna B"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["11908-11915"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10851674"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["94(34)"],"pubmed_abstract":["Although polymer-based lipid nanodiscs are increasingly used in the structural studies of membrane proteins, the charge of the belt-forming polymer is a major limitation for functional reconstitution of membrane proteins possessing an opposite net charge to that of the polymer. This limitation also rules out the reconstitution of a protein-protein complex composed of oppositely charged membrane proteins. In this study, we report the first successful functional reconstitution of a membrane-bound redox complex constituting a cationic cytochrome P450 (CYP450) and an anionic cytochrome P450 reductase (CPR) in non-ionic inulin-based lipid nanodiscs. The gel-to-liquid-crystalline phase-transition temperature (<i>T</i><sub>m</sub>) of DMPC:DMPG (7:3 w/w) lipids in polymer nanodiscs was determined"],"journal":["Analytical chemistry"],"pubmed_title":["Non-Ionic Inulin-Based Polymer Nanodiscs Enable Functional Reconstitution of a Redox Complex Composed of Oppositely Charged CYP450 and CPR in a Lipid Bilayer Membrane."],"pmcid":["PMC10851674"],"funding_grant_id":["R35GM139572","R35 GM139572","R35 GM139573"],"pubmed_authors":["Krishnarjuna B","Auchus RJ","Im SC","Marte J","Ravula T","Ramamoorthy A"],"additional_accession":[]},"is_claimable":false,"name":"Non-Ionic Inulin-Based Polymer Nanodiscs Enable Functional Reconstitution of a Redox Complex Composed of Oppositely Charged CYP450 and CPR in a Lipid Bilayer Membrane.","description":"Although polymer-based lipid nanodiscs are increasingly used in the structural studies of membrane proteins, the charge of the belt-forming polymer is a major limitation for functional reconstitution of membrane proteins possessing an opposite net charge to that of the polymer. This limitation also rules out the reconstitution of a protein-protein complex composed of oppositely charged membrane proteins. In this study, we report the first successful functional reconstitution of a membrane-bound redox complex constituting a cationic cytochrome P450 (CYP450) and an anionic cytochrome P450 reductase (CPR) in non-ionic inulin-based lipid nanodiscs. The gel-to-liquid-crystalline phase-transition temperature (<i>T</i><sub>m</sub>) of DMPC:DMPG (7:3 w/w) lipids in polymer nanodiscs was determined","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-19T18:31:54.113Z","creation":"2025-04-19T18:31:54.113Z"},"accession":"S-EPMC10851674","cross_references":{"pubmed":["35977417"],"doi":["10.1021/acs.analchem.2c02489"]}}