biostudies-literatureAhmed TAZewail City of Science &amp; TechnologySTDFEKB, STDF36https://www.ebi.ac.uk/biostudies/studies/S-EPMC10854071biostudies-literatureUnknown15(1)<h4>Background</h4>Pericytes (PCs) are multipotent contractile cells that wrap around the endothelial cells (ECs) to maintain the blood vessel's functionality and integrity. The hyperglycemia associated with Type 2 diabetes mellitus (T2DM) was shown to impair the function of PCs and increase the risk of diabetes complications. In this study, we aimed to investigate the deleterious effect of the diabetic microenvironment on the regenerative capacities of human PCs.<h4>Methods</h4>PCs isolated from human adipose tissue were cultured in the presence or absence of serum collected from diabetic patients. The functionality of PCs was analyzed after 6, 14, and 30 days.<h4>Results</h4>Microscopic examination of PCs cultured in DS (DS-PCs) showed increased aggregate formation and altered surface toStem cell research & therapyThe cross talk between type II diabetic microenvironment and the regenerative capacities of human adipose tissue-derived pericytes: a promising cell therapy.PMC10854071#5300FLUG 46721Open access fundingEl-Desouky MAOsama AEl-Badri NAhmed SMAnwar AMMohamed IKAhmed TAMagdeldin SHanna DHAbdelgawad MEElkhenany HfalseThe cross talk between type II diabetic microenvironment and the regenerative capacities of human adipose tissue-derived pericytes: a promising cell therapy.<h4>Background</h4>Pericytes (PCs) are multipotent contractile cells that wrap around the endothelial cells (ECs) to maintain the blood vessel's functionality and integrity. The hyperglycemia associated with Type 2 diabetes mellitus (T2DM) was shown to impair the function of PCs and increase the risk of diabetes complications. In this study, we aimed to investigate the deleterious effect of the diabetic microenvironment on the regenerative capacities of human PCs.<h4>Methods</h4>PCs isolated from human adipose tissue were cultured in the presence or absence of serum collected from diabetic patients. The functionality of PCs was analyzed after 6, 14, and 30 days.<h4>Results</h4>Microscopic examination of PCs cultured in DS (DS-PCs) showed increased aggregate formation and altered surface to2024-01-01T00:00:00Z2024 Feb2026-06-17T06:31:22.358Z2025-02-18T23:23:52.459ZS-EPMC108540713833188910.1186/s13287-024-03643-1