{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Demeler B"],"funding":["Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","National Institutes of Health","NIH HHS","NIGMS NIH HHS"],"pagination":["e974"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10857736"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(2)"],"pubmed_abstract":["Analytical ultracentrifugation experiments play an integral role in the solution-phase characterization of biological macromolecules and their interactions. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Proteomelab XL-A or XL-I analytical ultracentrifuge and with a Beckman Optima AUC. Instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments. © 2024 The Authors. Current Protocols publishe"],"journal":["Current protocols"],"pubmed_title":["Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments."],"pmcid":["PMC10857736"],"funding_grant_id":["GM‐120600","R01 GM120600","DG‐RGPIN‐2019‐05637","GM-120600"],"pubmed_authors":["Demeler B"],"additional_accession":[]},"is_claimable":false,"name":"Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments.","description":"Analytical ultracentrifugation experiments play an integral role in the solution-phase characterization of biological macromolecules and their interactions. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Proteomelab XL-A or XL-I analytical ultracentrifuge and with a Beckman Optima AUC. Instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments. © 2024 The Authors. Current Protocols publishe","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-05T11:34:06.037Z","creation":"2025-04-05T11:34:06.037Z"},"accession":"S-EPMC10857736","cross_references":{"pubmed":["38319042"],"doi":["10.1002/cpz1.974"]}}