biostudies-literatureLi DJiangsu Planned Projects for Postdoctoral Research FundsNational Natural Science Foundation of Chinathe Natural Science Foundation of the Jiangsu Higher Education Institutions of ChinaNatural Science Foundation of Jiangsu Province6https://www.ebi.ac.uk/biostudies/studies/S-EPMC10870677biostudies-literatureUnknown12(1)<h4>Background</h4>Metastasis is the leading cause of death among prostate cancer (PCa) patients. Obesity is associated with both PCa-specific and all-cause mortality. High-fat diet (HFD) is a risk factor contributing to obesity. However, the association of HFD with PCa metastasis and its underlying mechanisms are unclear.<h4>Methods</h4>Tumor xenografts were conducted by intrasplenic injections. The ability of migration or invasion was detected by transwell assay. The expression levels of RPS27 were detected by QRT-PCR and western blot.<h4>Results</h4>The present study verified the increase in PCa metastasis caused by HFD in mice. Bioinformatics analysis demonstrated increased RPS27 in the experimentally induced PCa in HFD mice, indicating that it is an unfavorable prognostic factor. IntrCancer & metabolismHigh-fat diet promotes prostate cancer metastasis via RPS27.PMC108706778207321222KJA320009BK20210909BK202206692021K431CLiang CZheng JMa BLi DCai YTang TYang TZhou XXu WWei LMu CZhao XfalseHigh-fat diet promotes prostate cancer metastasis via RPS27.<h4>Background</h4>Metastasis is the leading cause of death among prostate cancer (PCa) patients. Obesity is associated with both PCa-specific and all-cause mortality. High-fat diet (HFD) is a risk factor contributing to obesity. However, the association of HFD with PCa metastasis and its underlying mechanisms are unclear.<h4>Methods</h4>Tumor xenografts were conducted by intrasplenic injections. The ability of migration or invasion was detected by transwell assay. The expression levels of RPS27 were detected by QRT-PCR and western blot.<h4>Results</h4>The present study verified the increase in PCa metastasis caused by HFD in mice. Bioinformatics analysis demonstrated increased RPS27 in the experimentally induced PCa in HFD mice, indicating that it is an unfavorable prognostic factor. Intr2024-01-01T00:00:00Z2024 Feb2026-07-15T04:44:34.578Z2025-02-18T23:15:35.143ZS-EPMC108706773836577110.1186/s40170-024-00333-7