{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Bai Z"],"funding":["NIDDK NIH HHS","NIA NIH HHS","NIMH NIH HHS","NHGRI NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but as of today is largely limited to gene expression analysis. Yet, the life of RNA molecules is multifaceted and dynamic, requiring spatial profiling of different RNA species throughout the life cycle to delve into the intricate RNA biology in complex tissues. Human disease-relevant tissues are commonly preserved as formalin-fixed and paraffin-embedded (FFPE) blocks, representing an important resource for human tissue specimens. The capability to spatially explore RNA biology in FFPE tissues holds transformative potential for human biology research and clinical histopathology. Here, we present Patho-DBiT combining <i>in situ</i> polyadenylation and deterministic barcoding for spatial full"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.02.06.579143"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10871202"],"repository":["biostudies-literature"],"pubmed_title":["Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues."],"pmcid":["PMC10871202"],"funding_grant_id":["R56 HG012310","RF1 MH128876","RM1 MH132648","U54 AG079759","UM1 MH130991","R01 GM138856","U54 AG076043","UH3 CA257393","U54 CA268083","U54 DK106857","U54 CA274509","R01 CA245313","R33 CA246711"],"pubmed_authors":["Gerstein M","Zhang D","Xing Y","Xiao Y","Tao B","Zhang N","Bai Z","Bao S","Fan R","Su G","Li M","Xu ML","Liu Y","Tian X","Lu J","Gao Y","Enninful A"],"additional_accession":[]},"is_claimable":false,"name":"Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues.","description":"Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but as of today is largely limited to gene expression analysis. Yet, the life of RNA molecules is multifaceted and dynamic, requiring spatial profiling of different RNA species throughout the life cycle to delve into the intricate RNA biology in complex tissues. Human disease-relevant tissues are commonly preserved as formalin-fixed and paraffin-embedded (FFPE) blocks, representing an important resource for human tissue specimens. The capability to spatially explore RNA biology in FFPE tissues holds transformative potential for human biology research and clinical histopathology. Here, we present Patho-DBiT combining <i>in situ</i> polyadenylation and deterministic barcoding for spatial full","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2026-06-05T03:23:07.561Z","creation":"2026-06-05T03:11:52.593Z"},"accession":"S-EPMC10871202","cross_references":{"pubmed":["38370833"],"doi":["10.1101/2024.02.06.579143"]}}