biostudies-literatureUnknownGuzman SDIntramural NIH HHSNIA NIH HHSNIAMS NIH HHSThis investigation leverages single-cell RNA sequencing (scRNA-Seq) to delineate the contributions of muscle-resident Schwann cells to neuromuscular junction (NMJ) remodeling by comparing a model of stable innervation with models of reinnervation following partial or complete denervation. The study discovered multiple distinct Schwann cell subtypes, including a novel terminal Schwann cell (tSC) subtype integral to the denervation-reinnervation cycle, identified by a transcriptomic signature indicative of cell migration and polarization. The data also characterizes three myelin Schwann cell subtypes, which are distinguished based on enrichment of genes associated with myelin production, mesenchymal differentiation or collagen synthesis. Importantly, SPP1 signaling emerges as a pivotal regulbioRxiv : the preprint server for biology2023.10.06.561193https://www.ebi.ac.uk/biostudies/studies/S-EPMC10871306biostudies-literatureDecoding muscle-resident Schwann cell dynamics during neuromuscular junction remodeling.PMC10871306R01 AG086251Z01 AG000114R01 AG050676P01 AG051442P30 AR069620T32 AG000114Macpherson PCDGuzman SDRuiz LPDavis CSBrooks SVAbu-Mahfouz AfalseDecoding muscle-resident Schwann cell dynamics during neuromuscular junction remodeling.This investigation leverages single-cell RNA sequencing (scRNA-Seq) to delineate the contributions of muscle-resident Schwann cells to neuromuscular junction (NMJ) remodeling by comparing a model of stable innervation with models of reinnervation following partial or complete denervation. The study discovered multiple distinct Schwann cell subtypes, including a novel terminal Schwann cell (tSC) subtype integral to the denervation-reinnervation cycle, identified by a transcriptomic signature indicative of cell migration and polarization. The data also characterizes three myelin Schwann cell subtypes, which are distinguished based on enrichment of genes associated with myelin production, mesenchymal differentiation or collagen synthesis. Importantly, SPP1 signaling emerges as a pivotal regul2025-01-01T00:00:00Z2025 May2026-04-23T03:13:45.082Z2026-04-23T03:10:02.648ZS-EPMC108713063837085310.1101/2023.10.06.561193