{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sasaki T"],"funding":["MEXT | Japan Society for the Promotion of Science","Joint Usage and Joint Research Program of the Institute of Advanced Medical Sciences at Tokushima University, and the Institute for Molecular and Cellular Regulation at Gunma University","Takeda Science Foundation","NIH HHS"],"pagination":["1460"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10874384"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Allophagy is responsible for the selective removal of paternally inherited organelles, including mitochondria, in Caenorhabditis elegans embryos, thereby facilitating the maternal inheritance of mitochondrial DNA. We previously identified two key factors in allophagy: an autophagy adaptor allophagy-1 (ALLO-1) and TBK1/IKKε family kinase IKKE-1. However, the precise mechanisms by which ALLO-1 and IKKE-1 regulate local autophagosome formation remain unclear. In this study, we identify two ALLO-1 isoforms with different substrate preferences during allophagy. Live imaging reveals a stepwise mechanism of ALLO-1 localization with rapid cargo recognition, followed by ALLO-1 accumulation around the cargo. In the ikke-1 mutant, the accumulation of ALLO-1, and not the recognition of cargo, is impai"],"journal":["Nature communications"],"pubmed_title":["ALLO-1- and IKKE-1-dependent positive feedback mechanism promotes the initiation of paternal mitochondrial autophagy."],"pmcid":["PMC10874384"],"funding_grant_id":["21H02472","20J01777","22KJ0444","19H05712","19H05711","22K15097","20H00466","P40 OD010440"],"pubmed_authors":["Kushida Y","Norizuki T","Sato M","Sasaki T","Sato K","Kosako H"],"additional_accession":[]},"is_claimable":false,"name":"ALLO-1- and IKKE-1-dependent positive feedback mechanism promotes the initiation of paternal mitochondrial autophagy.","description":"Allophagy is responsible for the selective removal of paternally inherited organelles, including mitochondria, in Caenorhabditis elegans embryos, thereby facilitating the maternal inheritance of mitochondrial DNA. We previously identified two key factors in allophagy: an autophagy adaptor allophagy-1 (ALLO-1) and TBK1/IKKε family kinase IKKE-1. However, the precise mechanisms by which ALLO-1 and IKKE-1 regulate local autophagosome formation remain unclear. In this study, we identify two ALLO-1 isoforms with different substrate preferences during allophagy. Live imaging reveals a stepwise mechanism of ALLO-1 localization with rapid cargo recognition, followed by ALLO-1 accumulation around the cargo. In the ikke-1 mutant, the accumulation of ALLO-1, and not the recognition of cargo, is impai","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2026-07-14T18:54:29.813Z","creation":"2025-04-05T00:29:40.671Z"},"accession":"S-EPMC10874384","cross_references":{"pubmed":["38368448"],"doi":["10.1038/s41467-024-45863-2"]}}