biostudies-literatureHu Rthe Science and Technology Research Program of Chongqing Municipal Education CommissionNational Science Foundation of Chinathe fellowship of China Postdoctoral Science Foundationthe Scientific Research Foundation of the Science and Technology Commission of ChongqingNational Key Research and Development Program of China177https://www.ebi.ac.uk/biostudies/studies/S-EPMC10874557biostudies-literatureUnknown22(1)<h4>Background</h4>Human health is seriously threatened by antibiotic-induced intestinal disorders. Herein, we aimed to determine the effects of Autoinducer-2 (AI-2) combined with Lactobacillus rhamnosus GG (LGG) on the intestinal barrier function of antibiotic-induced intestinal dysbiosis neonatal mice.<h4>Methods</h4>An antibiotic-induced intestinal dysbiosis neonatal mouse model was created using antibiotic cocktails, and the model mice were randomized into the control, AI-2, LGG, and LGG + AI-2 groups. Intestinal short-chain fatty acids and AI-2 concentrations were detected by mass spectrometry and chemiluminescence, respectively. The community composition of the gut microbiota was analyzed using 16S rDNA sequencing, and biofilm thickness and bacterial adhesion in the colon were assessJournal of translational medicineAutoinducer-2 promotes the colonization of Lactobacillus rhamnosus GG to improve the intestinal barrier function in a neonatal mouse model of antibiotic-induced intestinal dysbiosis.PMC10874557319710892022YFC27048022022MD713716KJQN202200414CSTB2023NSCQ-MSX0178Tong BSun QChen JAi QJi YYang THu RWang ZShi YfalseAutoinducer-2 promotes the colonization of Lactobacillus rhamnosus GG to improve the intestinal barrier function in a neonatal mouse model of antibiotic-induced intestinal dysbiosis.<h4>Background</h4>Human health is seriously threatened by antibiotic-induced intestinal disorders. Herein, we aimed to determine the effects of Autoinducer-2 (AI-2) combined with Lactobacillus rhamnosus GG (LGG) on the intestinal barrier function of antibiotic-induced intestinal dysbiosis neonatal mice.<h4>Methods</h4>An antibiotic-induced intestinal dysbiosis neonatal mouse model was created using antibiotic cocktails, and the model mice were randomized into the control, AI-2, LGG, and LGG + AI-2 groups. Intestinal short-chain fatty acids and AI-2 concentrations were detected by mass spectrometry and chemiluminescence, respectively. The community composition of the gut microbiota was analyzed using 16S rDNA sequencing, and biofilm thickness and bacterial adhesion in the colon were assess2024-01-01T00:00:00Z2024 Feb2026-06-18T04:45:03.296Z2025-04-05T23:49:51.346ZS-EPMC108745573836950310.1186/s12967-024-04991-5