biostudies-literatureUnknown15Zhou XExtracellular enzymes play important roles in myxobacteria degrading macromolecules and preying on other microorganisms. Glycoside hydrolases 19 (GH19) are widely present in myxobacteria, but their evolution and biological functions have not been fully elucidated. Here we investigated the comparative secretory proteome of <i>Corallococcus silvisoli</i> c25j21 in the presence of cellulose and chitin. A total of 313 proteins were detected, including 16 carbohydrate-active enzymes (CAZymes), 7 of which were induced by cellulose or chitin, such as GH6, GH13, GH19, AA4, and CBM56. We further analyzed the sequence and structural characteristics of its three GH19 enzymes to understand their potential functions. The results revealed that myxobacterial GH19 enzymes are evolutionarily divided into tFrontiers in microbiology1324153https://www.ebi.ac.uk/biostudies/studies/S-EPMC10875029biostudies-literatureSecretory CAZymes profile and GH19 enzymes analysis of <i>Corallococcus silvisoli</i> c25j21.PMC10875029Dong YDong HZhang XZhu HZhou XfalseSecretory CAZymes profile and GH19 enzymes analysis of <i>Corallococcus silvisoli</i> c25j21.Extracellular enzymes play important roles in myxobacteria degrading macromolecules and preying on other microorganisms. Glycoside hydrolases 19 (GH19) are widely present in myxobacteria, but their evolution and biological functions have not been fully elucidated. Here we investigated the comparative secretory proteome of <i>Corallococcus silvisoli</i> c25j21 in the presence of cellulose and chitin. A total of 313 proteins were detected, including 16 carbohydrate-active enzymes (CAZymes), 7 of which were induced by cellulose or chitin, such as GH6, GH13, GH19, AA4, and CBM56. We further analyzed the sequence and structural characteristics of its three GH19 enzymes to understand their potential functions. The results revealed that myxobacterial GH19 enzymes are evolutionarily divided into t2024-01-01T00:00:00Z20242026-04-21T03:23:01.42Z2025-04-19T07:35:23.924ZS-EPMC108750293837491410.3389/fmicb.2024.1324153