biostudies-literatureDiaz de Leon JSANIAID NIH HHSNational Institutes of HealthNIH HHS1182-1192https://www.ebi.ac.uk/biostudies/studies/S-EPMC10876040biostudies-literatureUnknown33(12)Factors regulating macrophage effector function represent potential targets to optimize the efficacy of antibody-mediated therapies. Macrophages are myeloid cells capable of engulfing and destroying diseased or damaged target cells. Antibodies binding to the target cell surface can engage macrophage Fc gamma receptors (FcγRs) to elicit antibody-dependent cellular phagocytosis (ADCP), a process that contributes to treatments mediated by anti-tumor antibodies. Conversely, macrophage ADCP of apoptotic T cells is also linked to tolerance in the tumor environment. Here we evaluated the role of asparagine(N)-linked glycans in the function of macrophages derived from primary human monocytes. Macrophages treated with kifunensine, an inhibitor of N-glycan processing, exhibited greater target bindinGlycobiologyMacrophage N-glycan processing inhibits antibody-dependent cellular phagocytosis.PMC10876040U01 AI148114Aguilar IBarb AWDiaz de Leon JSAfalseMacrophage N-glycan processing inhibits antibody-dependent cellular phagocytosis.Factors regulating macrophage effector function represent potential targets to optimize the efficacy of antibody-mediated therapies. Macrophages are myeloid cells capable of engulfing and destroying diseased or damaged target cells. Antibodies binding to the target cell surface can engage macrophage Fc gamma receptors (FcγRs) to elicit antibody-dependent cellular phagocytosis (ADCP), a process that contributes to treatments mediated by anti-tumor antibodies. Conversely, macrophage ADCP of apoptotic T cells is also linked to tolerance in the tumor environment. Here we evaluated the role of asparagine(N)-linked glycans in the function of macrophages derived from primary human monocytes. Macrophages treated with kifunensine, an inhibitor of N-glycan processing, exhibited greater target bindin2023-01-01T00:00:00Z2023 Dec2025-04-04T01:03:17.002Z2025-04-04T01:03:17.002ZS-EPMC108760403779285710.1093/glycob/cwad078