biostudies-literatureUnknown130(4)Mezquita L<h4>Background</h4>Resistance mechanisms to combination therapy with dabrafenib plus trametinib remain poorly understood in patients with BRAF^V600E-mutant advanced non-small-cell lung cancer (NSCLC). We examined resistance to BRAF inhibition by single CTC sequencing in BRAF^V600E-mutant NSCLC.<h4>Methods</h4>CTCs and cfDNA were examined in seven BRAF^V600E-mutant NSCLC patients at failure to treatment. Matched tumour tissue was available for four patients. Single CTCs were isolated by fluorescence-activated cell sorting following enrichment and immunofluorescence (Hoechst 33342/CD45/pan-cytokeratins) and sequenced for mutation and copy number-alteration (CNA) analyses.<h4>Results</h4>BRAF^V600E was found in 4/4 tumour biopsies and 5/7 cfDNA samples.British journal of cancer682-693https://www.ebi.ac.uk/biostudies/studies/S-EPMC10876548biostudies-literatureResistance to BRAF inhibition explored through single circulating tumour cell molecular profiling in BRAF-mutant non-small-cell lung cancer.PMC10876548Oulhen MAldea MHonore AFriboulet LMezquita LDeloger MPlanchard DHowarth KBesse BLecluse YAberlenc AFarace FfalseResistance to BRAF inhibition explored through single circulating tumour cell molecular profiling in BRAF-mutant non-small-cell lung cancer.<h4>Background</h4>Resistance mechanisms to combination therapy with dabrafenib plus trametinib remain poorly understood in patients with BRAF^V600E-mutant advanced non-small-cell lung cancer (NSCLC). We examined resistance to BRAF inhibition by single CTC sequencing in BRAF^V600E-mutant NSCLC.<h4>Methods</h4>CTCs and cfDNA were examined in seven BRAF^V600E-mutant NSCLC patients at failure to treatment. Matched tumour tissue was available for four patients. Single CTCs were isolated by fluorescence-activated cell sorting following enrichment and immunofluorescence (Hoechst 33342/CD45/pan-cytokeratins) and sequenced for mutation and copy number-alteration (CNA) analyses.<h4>Results</h4>BRAF^V600E was found in 4/4 tumour biopsies and 5/7 cfDNA samples.2024-01-01T00:00:00Z2024 Mar2025-04-22T11:05:49.335Z2025-04-05T23:52:24.121ZS-EPMC108765483817766010.1038/s41416-023-02535-0