{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["Pucelik S"],"pubmed_abstract":["In the marine α-proteobacterium <i>Dinoroseobacter shibae</i> more than 40 genes of the aerobic anoxygenic photosynthesis are regulated in a light-dependent manner. A genome-wide screen of 5,605 clones from a <i>D. shibae</i> transposon library for loss of pigmentation and changes in bacteriochlorophyll absorbance identified 179 mutant clones. The gene encoding the LOV-domain containing protein Dshi_1135 was identified by its colorless phenotype. The mutant phenotype was complemented by the expression of a Dshi_1135-strep fusion protein in trans. The recombinantly produced and chromatographically purified Dshi_1135 protein was able to undergo a blue light-induced photocycle mediated by bound FMN. Transcriptome analyses revealed an essential role for Dshi_1135 in the light-dependent express"],"journal":["Frontiers in microbiology"],"pagination":["1351297"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10890935"],"repository":["biostudies-literature"],"pubmed_title":["The blue light-dependent LOV-protein LdaP of <i>Dinoroseobacter shibae</i> acts as antirepressor of the PpsR repressor, regulating photosynthetic gene cluster expression."],"pmcid":["PMC10890935"],"pubmed_authors":["Becker M","Rabus R","Hartig E","Wohlbrand L","Pucelik S","Heyber S","Jahn D"],"additional_accession":[]},"is_claimable":false,"name":"The blue light-dependent LOV-protein LdaP of <i>Dinoroseobacter shibae</i> acts as antirepressor of the PpsR repressor, regulating photosynthetic gene cluster expression.","description":"In the marine α-proteobacterium <i>Dinoroseobacter shibae</i> more than 40 genes of the aerobic anoxygenic photosynthesis are regulated in a light-dependent manner. A genome-wide screen of 5,605 clones from a <i>D. shibae</i> transposon library for loss of pigmentation and changes in bacteriochlorophyll absorbance identified 179 mutant clones. The gene encoding the LOV-domain containing protein Dshi_1135 was identified by its colorless phenotype. The mutant phenotype was complemented by the expression of a Dshi_1135-strep fusion protein in trans. The recombinantly produced and chromatographically purified Dshi_1135 protein was able to undergo a blue light-induced photocycle mediated by bound FMN. Transcriptome analyses revealed an essential role for Dshi_1135 in the light-dependent express","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-07-15T09:59:47.66Z","creation":"2025-04-19T21:06:37.036Z"},"accession":"S-EPMC10890935","cross_references":{"pubmed":["38404597"],"doi":["10.3389/fmicb.2024.1351297"]}}