biostudies-literatureUnknown15Pucelik SIn the marine α-proteobacterium <i>Dinoroseobacter shibae</i> more than 40 genes of the aerobic anoxygenic photosynthesis are regulated in a light-dependent manner. A genome-wide screen of 5,605 clones from a <i>D. shibae</i> transposon library for loss of pigmentation and changes in bacteriochlorophyll absorbance identified 179 mutant clones. The gene encoding the LOV-domain containing protein Dshi_1135 was identified by its colorless phenotype. The mutant phenotype was complemented by the expression of a Dshi_1135-strep fusion protein in trans. The recombinantly produced and chromatographically purified Dshi_1135 protein was able to undergo a blue light-induced photocycle mediated by bound FMN. Transcriptome analyses revealed an essential role for Dshi_1135 in the light-dependent expressFrontiers in microbiology1351297https://www.ebi.ac.uk/biostudies/studies/S-EPMC10890935biostudies-literatureThe blue light-dependent LOV-protein LdaP of <i>Dinoroseobacter shibae</i> acts as antirepressor of the PpsR repressor, regulating photosynthetic gene cluster expression.PMC10890935Becker MRabus RHartig EWohlbrand LPucelik SHeyber SJahn DfalseThe blue light-dependent LOV-protein LdaP of <i>Dinoroseobacter shibae</i> acts as antirepressor of the PpsR repressor, regulating photosynthetic gene cluster expression.In the marine α-proteobacterium <i>Dinoroseobacter shibae</i> more than 40 genes of the aerobic anoxygenic photosynthesis are regulated in a light-dependent manner. A genome-wide screen of 5,605 clones from a <i>D. shibae</i> transposon library for loss of pigmentation and changes in bacteriochlorophyll absorbance identified 179 mutant clones. The gene encoding the LOV-domain containing protein Dshi_1135 was identified by its colorless phenotype. The mutant phenotype was complemented by the expression of a Dshi_1135-strep fusion protein in trans. The recombinantly produced and chromatographically purified Dshi_1135 protein was able to undergo a blue light-induced photocycle mediated by bound FMN. Transcriptome analyses revealed an essential role for Dshi_1135 in the light-dependent express2024-01-01T00:00:00Z20242026-07-15T09:59:47.66Z2025-04-19T21:06:37.036ZS-EPMC108909353840459710.3389/fmicb.2024.1351297