biostudies-literatureIsmail JDeutsche Forschungsgemeinschaft187https://www.ebi.ac.uk/biostudies/studies/S-EPMC10891717biostudies-literatureUnknown16(2)Hybrid nanoparticles (HNPs) were designed by combining a PLGA core with a lipid shell that incorporated PEG-Lipid conjugates with various functionalities (-RGD, -cRGD, -NH₂, and -COOH) to create targeted drug delivery systems. Loaded with a neutral lipid orange dye, the HNPs were extensively characterized using various techniques and investigated for their uptake in human monocyte-derived macrophages (MDMs) using FC and CLSM. Moreover, the best-performing HNPs (i.e., HNP-COOH and HNP-RGD as well as HNP-RGD/COOH mixed) were loaded with the anti-inflammatory drug BRP-201 and prepared in two size ranges (d_H ~140 nm and d_H ~250 nm). The HNPs were examined further for their stability, degradation, MDM uptake, and drug delivery efficiency by studying the inhibitiPharmaceuticsPEG-Lipid-PLGA Hybrid Particles for Targeted Delivery of Anti-Inflammatory Drugs.PMC10891717471397362316213987INST 275/315-1 FUGGIsmail JPretzel DRezaei KNischang IDahlke PCzaplewska JAJordan PMSchubert USBeringer-Siemers BTsarenko EStumpf SVollrath AKlepsch LCHoeppener SWerz OfalsePEG-Lipid-PLGA Hybrid Particles for Targeted Delivery of Anti-Inflammatory Drugs.Hybrid nanoparticles (HNPs) were designed by combining a PLGA core with a lipid shell that incorporated PEG-Lipid conjugates with various functionalities (-RGD, -cRGD, -NH₂, and -COOH) to create targeted drug delivery systems. Loaded with a neutral lipid orange dye, the HNPs were extensively characterized using various techniques and investigated for their uptake in human monocyte-derived macrophages (MDMs) using FC and CLSM. Moreover, the best-performing HNPs (i.e., HNP-COOH and HNP-RGD as well as HNP-RGD/COOH mixed) were loaded with the anti-inflammatory drug BRP-201 and prepared in two size ranges (d_H ~140 nm and d_H ~250 nm). The HNPs were examined further for their stability, degradation, MDM uptake, and drug delivery efficiency by studying the inhibiti2024-01-01T00:00:00Z2024 Jan2025-04-19T14:47:46.113Z2025-04-19T14:47:46.113ZS-EPMC108917173839924810.3390/pharmaceutics16020187