biostudies-literatureTian Qpecial fund for scientific and technological innovation of Fujian Agricultural and Forestry University Applied Basic ResearchNatural Science Foundation of Fujian ProvinceNational Natural Science Foundation of ChinaSpecial fund for scientific and technological innovation of Fujian Agricultural and Forestry University Applied Basic Researchstartup fund of Minnan Normal University480https://www.ebi.ac.uk/biostudies/studies/S-EPMC10891736biostudies-literatureUnknown13(4)Ran GTPases play essential roles in plant growth and development. Our previous studies revealed the nuclear localization of DlRan3A and DlRan3B proteins and proposed their functional redundancy and distinction in <i>Dimocarpus longan</i> somatic embryogenesis, hormone, and abiotic stress responses. To further explore the possible roles of <i>DlRan3A</i> and <i>DlRan3B</i>, gene expression analysis by qPCR showed that their transcripts were both more abundant in the early embryo and pulp in longan. Heterologous expression of <i>DlRan3A</i> driven by its own previously cloned promoter led to stunted growth, increased root hair density, abnormal fruits, bigger seeds, and enhanced abiotic stress tolerance. Conversely, constitutive promoter <i>CaMV 35S</i> (<i>35S</i>)-driven expression of <i>DPlants (Basel, Switzerland)Functional and Transcriptome Analysis Reveal Specific Roles of <i>Dimocarpus longan DlRan3A</i> and <i>DlRan3B</i> in Root Hair Development, Reproductive Growth, and Stress Tolerance.PMC1089173631672127CXZX2017189 and CXZX201731431572088CXZX20173142021J051992020N0061 and 2021J05199CXZX20171894206-L217152020N0061Cheng CLai ZXie XZhang ZLai RChen YLin YTian QXuHan XfalseFunctional and Transcriptome Analysis Reveal Specific Roles of <i>Dimocarpus longan DlRan3A</i> and <i>DlRan3B</i> in Root Hair Development, Reproductive Growth, and Stress Tolerance.Ran GTPases play essential roles in plant growth and development. Our previous studies revealed the nuclear localization of DlRan3A and DlRan3B proteins and proposed their functional redundancy and distinction in <i>Dimocarpus longan</i> somatic embryogenesis, hormone, and abiotic stress responses. To further explore the possible roles of <i>DlRan3A</i> and <i>DlRan3B</i>, gene expression analysis by qPCR showed that their transcripts were both more abundant in the early embryo and pulp in longan. Heterologous expression of <i>DlRan3A</i> driven by its own previously cloned promoter led to stunted growth, increased root hair density, abnormal fruits, bigger seeds, and enhanced abiotic stress tolerance. Conversely, constitutive promoter <i>CaMV 35S</i> (<i>35S</i>)-driven expression of <i>D2024-01-01T00:00:00Z2024 Feb2026-05-23T03:21:38.068Z2025-05-29T22:00:47.755ZS-EPMC108917363849844410.3390/plants13040480