biostudies-literatureZhu MNational Guidance Foundation for Local Science and Technology Development of ChinaGuizhou Provincial Key Technology R&amp;amp;D ProgramNatural Science Special Research Fund of Guizhou UniversityGuizhou Provincial Basic Research Program (Natural Science)National Natural Science Foundation of ChinaGuizhou Provincial Key Technology R&D ProgramFoundation of Key Laboratory of Animal Genetics, Breeding and Reproduction in The Plateau Mountainous Region, Ministry of Education, Guizhou University855https://www.ebi.ac.uk/biostudies/studies/S-EPMC10891758biostudies-literatureUnknown29(4)Deoxynivalenol (DON) is a common mycotoxin that is widely found in various foods and feeds, posing a potential threat to human and animal health. This study aimed to investigate the protective effect of the natural polyphenol piceatannol (PIC) against DON-induced damage in porcine intestinal epithelial cells (IPEC-J2 cells) and the underlying mechanism. The results showed that PIC promotes IPEC-J2 cell proliferation in a dose-dependent manner. Moreover, it not only significantly relieved DON-induced decreases in cell viability and proliferation but also reduced intracellular reactive oxygen species (ROS) production. Further studies demonstrated that PIC alleviated DON-induced oxidative stress damage by increasing the protein expression levels of the antioxidant factors NAD(P)H quinone oxidoreductase-1 (NQO1) and glutamate-cysteine ligase modifier subunit (GCLM), and the mRNA expression of <i>catalase</i> (<i>CAT</i>), <i>Superoxide Dismutase 1</i> (<i>SOD1</i>), <i>peroxiredoxin 3</i> (<i>PRX3</i>), and <i>glutathione S-transferase alpha 4</i> (<i>GSTα4</i>). In addition, PIC inhibited the activation of the nuclear factor-B (NF-κB) pathway, downregulated the mRNA expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) to attenuate DON-induced inflammatory responses, and further mitigated DON-induced cellular intestinal barrier injury by regulating the protein expression of Occludin. These findings indicated that PIC had a significant protective effect against DON-induced damage. This study provides more understanding to support PIC as a feed additive for pig production.Molecules (Basel, Switzerland)Piceatannol Alleviates Deoxynivalenol-Induced Damage in Intestinal Epithelial Cells via Inhibition of the NF-κB Pathway.PMC1089175832202710Qiankehe foundation-ZK [2022] General 1592023-009QJH-KY [2020] 257QKHZC-2021-General 147Gui Da Te Gang He Zi [2020] 24Zhu MZhang YYLiu GWCheng YJLu EQHuang KXu EFang YXWang XJfalsePiceatannol Alleviates Deoxynivalenol-Induced Damage in Intestinal Epithelial Cells via Inhibition of the NF-κB Pathway.Deoxynivalenol (DON) is a common mycotoxin that is widely found in various foods and feeds, posing a potential threat to human and animal health. This study aimed to investigate the protective effect of the natural polyphenol piceatannol (PIC) against DON-induced damage in porcine intestinal epithelial cells (IPEC-J2 cells) and the underlying mechanism. The results showed that PIC promotes IPEC-J2 cell proliferation in a dose-dependent manner. Moreover, it not only significantly relieved DON-induced decreases in cell viability and proliferation but also reduced intracellular reactive oxygen species (ROS) production. Further studies demonstrated that PIC alleviated DON-induced oxidative stress damage by increasing the protein expression levels of the antioxidant factors NAD(P)H quinone oxidoreductase-1 (NQO1) and glutamate-cysteine ligase modifier subunit (GCLM), and the mRNA expression of <i>catalase</i> (<i>CAT</i>), <i>Superoxide Dismutase 1</i> (<i>SOD1</i>), <i>peroxiredoxin 3</i> (<i>PRX3</i>), and <i>glutathione S-transferase alpha 4</i> (<i>GSTα4</i>). In addition, PIC inhibited the activation of the nuclear factor-B (NF-κB) pathway, downregulated the mRNA expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) to attenuate DON-induced inflammatory responses, and further mitigated DON-induced cellular intestinal barrier injury by regulating the protein expression of Occludin. These findings indicated that PIC had a significant protective effect against DON-induced damage. This study provides more understanding to support PIC as a feed additive for pig production.2024-01-01T00:00:00Z2024 Feb2026-07-03T03:15:06.822Z2026-07-03T03:10:17.298ZS-EPMC108917583839860710.3390/molecules29040855