biostudies-literatureLi LSichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal UniversityRunlan WanCooperative Project between Dazhou City and Dazhou Vocational College of Chinese MedicineQin WangPhoenix Project from Dazhou CityChun ZhangHuairong LuoPostdoctoral Research Initiation Fund from Southwest Medical UniversityScience and Technology Project of Luzhou CityScience &amp; Technology Program from Sichuan Province908https://www.ebi.ac.uk/biostudies/studies/S-EPMC10891841biostudies-literatureUnknown29(4)<i>Dendrobium nobile</i> is a traditional Chinese herb with anti-inflammatory, antioxidant, and neuroprotective properties. However, its antiaging effects are unclear. Herein, we studied the aging-related functions and the mechanism of action of the alcohol extract of <i>Dendrobium nobile</i> (DnAE) in the model organism <i>Caenorhabditis elegans</i>. The results indicated that 1 mg/mL DnAE slowed lipofuscin accumulation, decreased the levels of reactive oxygen species, elevated superoxide dismutase activity, enhanced oxidative and heat stress resistance, extended the lifespan of nematodes, protected their dopamine neurons from 6-hydroxydopamine-induced neurodegeneration, and reduced Aβ-induced neurotoxicity. DnAE upregulated the mRNA expression of the transcription factors DAF-16 and HSF-Molecules (Basel, Switzerland)Dendrobium Nobile Alcohol Extract Extends the Lifespan of <i>Caenorhabditis elegans</i> via <i>hsf-1</i> and <i>daf-16</i>.PMC108918412023JYJ051Grant No. 02/00170043Grant No. SCSJ20210092022SWMU42022-SYF-632023TSYY-05DZFHXM2023182021YJ0114DZKJJ2022S042021-SYF-3502/00170043Grant No. 2021YJ0114Grant No. 2022-SYF-63Cai RWan RLiu ZWang QHu HLuo HZhang CLi LZhou XBi JfalseDendrobium Nobile Alcohol Extract Extends the Lifespan of <i>Caenorhabditis elegans</i> via <i>hsf-1</i> and <i>daf-16</i>.<i>Dendrobium nobile</i> is a traditional Chinese herb with anti-inflammatory, antioxidant, and neuroprotective properties. However, its antiaging effects are unclear. Herein, we studied the aging-related functions and the mechanism of action of the alcohol extract of <i>Dendrobium nobile</i> (DnAE) in the model organism <i>Caenorhabditis elegans</i>. The results indicated that 1 mg/mL DnAE slowed lipofuscin accumulation, decreased the levels of reactive oxygen species, elevated superoxide dismutase activity, enhanced oxidative and heat stress resistance, extended the lifespan of nematodes, protected their dopamine neurons from 6-hydroxydopamine-induced neurodegeneration, and reduced Aβ-induced neurotoxicity. DnAE upregulated the mRNA expression of the transcription factors DAF-16 and HSF-2024-01-01T00:00:00Z2024 Feb2026-07-06T03:15:14.564Z2025-04-19T14:46:21.659ZS-EPMC108918413839865810.3390/molecules29040908