biostudies-literatureSvoboda TAustrian Science Fund FWFAustrian Research Promotion AgencyNFB99https://www.ebi.ac.uk/biostudies/studies/S-EPMC10893509biostudies-literatureUnknown16(2)<i>Fusarium</i> is a genus that mostly consists of plant pathogenic fungi which are able to produce a broad range of toxic secondary metabolites. In this study, we focus on a type A trichothecene-producing isolate (15-39) of <i>Fusarium sporotrichioides</i> from Lower Austria. We assessed the secondary metabolite profile and optimized the toxin production conditions on autoclaved rice and found that in addition to large amounts of T-2 and HT-2 toxins, this strain was able to produce HT-2-glucoside. The optimal conditions for the production of T-2 toxin, HT-2 toxin, and HT-2-glucoside on autoclaved rice were incubation at 12 °C under constant light for four weeks, darkness at 30 °C for two weeks, and constant light for three weeks at 20 °C, respectively. The HT-2-glucoside was purified, andToxins<i>Fusarium sporotrichioides</i> Produces Two HT-2-α-Glucosides on Rice.PMC10893509F3708COMET-K1 Competence Centre FFoQSIBiMM K3-G-2/08-2020Krska RLabuda RBerthiller FSulyok MBacher MSvoboda TAdam Gfalse<i>Fusarium sporotrichioides</i> Produces Two HT-2-α-Glucosides on Rice.<i>Fusarium</i> is a genus that mostly consists of plant pathogenic fungi which are able to produce a broad range of toxic secondary metabolites. In this study, we focus on a type A trichothecene-producing isolate (15-39) of <i>Fusarium sporotrichioides</i> from Lower Austria. We assessed the secondary metabolite profile and optimized the toxin production conditions on autoclaved rice and found that in addition to large amounts of T-2 and HT-2 toxins, this strain was able to produce HT-2-glucoside. The optimal conditions for the production of T-2 toxin, HT-2 toxin, and HT-2-glucoside on autoclaved rice were incubation at 12 °C under constant light for four weeks, darkness at 30 °C for two weeks, and constant light for three weeks at 20 °C, respectively. The HT-2-glucoside was purified, and2024-01-01T00:00:00Z2024 Feb2026-06-29T03:11:27.268Z2025-04-07T03:40:22.993ZS-EPMC108935093839317710.3390/toxins16020099