{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Amato B"],"funding":["Ministero della Salute"],"pagination":["407"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10893549"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["Bovine tuberculosis and paratuberculosis are endemic in many areas worldwide. This work aims to study cytokines production and gene expression profiles of bovine macrophages infected with <i>Mycobacterium bovis</i> and <i>Mycobacterium paratuberculosis</i> subsp. <i>avium</i> (MAP) strains to identify potential diagnostic biomarkers. Bovine bone marrow stem cells were differentiated into macrophages and subsequently infected in vitro with different spoligotypes of <i>M. bovis</i> and MAP field strains (as single infections and coinfections), using different multiplicity of infection. Supernatant and cell pellets were collected 24 h, 48 h, and one week post-infection. Preliminarily, gene expression on cell pellets of IL-1β, IL-2, INFγ, IL-6, IL-10, IL-12, and TNFα was assessed by qRT-PCR on"],"journal":["Microorganisms"],"pubmed_title":["Comparative Study of <i>Mycobacterium bovis</i> and <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results."],"pmcid":["PMC10893549"],"funding_grant_id":["IZS SI 12/14 RC"],"pubmed_authors":["Amato B","Ippolito D","Pruiti Ciarello F","Alduina R","Gerace E","Cannella V","Fiasconaro M","Galluzzo P","Vitale M","Di Marco Lo Presti V"],"additional_accession":[]},"is_claimable":false,"name":"Comparative Study of <i>Mycobacterium bovis</i> and <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results.","description":"Bovine tuberculosis and paratuberculosis are endemic in many areas worldwide. This work aims to study cytokines production and gene expression profiles of bovine macrophages infected with <i>Mycobacterium bovis</i> and <i>Mycobacterium paratuberculosis</i> subsp. <i>avium</i> (MAP) strains to identify potential diagnostic biomarkers. Bovine bone marrow stem cells were differentiated into macrophages and subsequently infected in vitro with different spoligotypes of <i>M. bovis</i> and MAP field strains (as single infections and coinfections), using different multiplicity of infection. Supernatant and cell pellets were collected 24 h, 48 h, and one week post-infection. Preliminarily, gene expression on cell pellets of IL-1β, IL-2, INFγ, IL-6, IL-10, IL-12, and TNFα was assessed by qRT-PCR on","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-22T13:26:13.416Z","creation":"2025-02-19T01:38:41.218Z"},"accession":"S-EPMC10893549","cross_references":{"pubmed":["38399810"],"doi":["10.3390/microorganisms12020407"]}}