biostudies-literatureAmato BMinistero della Salute407https://www.ebi.ac.uk/biostudies/studies/S-EPMC10893549biostudies-literatureUnknown12(2)Bovine tuberculosis and paratuberculosis are endemic in many areas worldwide. This work aims to study cytokines production and gene expression profiles of bovine macrophages infected with <i>Mycobacterium bovis</i> and <i>Mycobacterium paratuberculosis</i> subsp. <i>avium</i> (MAP) strains to identify potential diagnostic biomarkers. Bovine bone marrow stem cells were differentiated into macrophages and subsequently infected in vitro with different spoligotypes of <i>M. bovis</i> and MAP field strains (as single infections and coinfections), using different multiplicity of infection. Supernatant and cell pellets were collected 24 h, 48 h, and one week post-infection. Preliminarily, gene expression on cell pellets of IL-1β, IL-2, INFγ, IL-6, IL-10, IL-12, and TNFα was assessed by qRT-PCR onMicroorganismsComparative Study of <i>Mycobacterium bovis</i> and <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results.PMC10893549IZS SI 12/14 RCAmato BIppolito DPruiti Ciarello FAlduina RGerace ECannella VFiasconaro MGalluzzo PVitale MDi Marco Lo Presti VfalseComparative Study of <i>Mycobacterium bovis</i> and <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results.Bovine tuberculosis and paratuberculosis are endemic in many areas worldwide. This work aims to study cytokines production and gene expression profiles of bovine macrophages infected with <i>Mycobacterium bovis</i> and <i>Mycobacterium paratuberculosis</i> subsp. <i>avium</i> (MAP) strains to identify potential diagnostic biomarkers. Bovine bone marrow stem cells were differentiated into macrophages and subsequently infected in vitro with different spoligotypes of <i>M. bovis</i> and MAP field strains (as single infections and coinfections), using different multiplicity of infection. Supernatant and cell pellets were collected 24 h, 48 h, and one week post-infection. Preliminarily, gene expression on cell pellets of IL-1β, IL-2, INFγ, IL-6, IL-10, IL-12, and TNFα was assessed by qRT-PCR on2024-01-01T00:00:00Z2024 Feb2025-04-22T13:26:13.416Z2025-02-19T01:38:41.218ZS-EPMC108935493839981010.3390/microorganisms12020407