biostudies-literatureUnknown41(2)Harris ALBedford Research Foundation<h4>Objective</h4>This study is to discover hormone pathways active in early cleaving human embryos.<h4>Methods</h4>A list of 152 hormones and receptors were compiled to query the microarray database of mRNAs in 8-cell human embryos, two lines of human embryonic stem cells plus human fibroblasts before and after induced pluripotency.<h4>Results</h4>Over half of the 152 hormones and receptors were silent on the arrays of all cell types, and more were detected at high or moderate levels on the 8-cell arrays than on the pluripotent cell or fibroblast arrays. Eight hormone family genes were uniquely detected at least 22-fold higher on the 8-cell arrays than the stem cell arrays: AVPI1, CCK, CORT, FSTL4, GIP, GPHA2, OXT, and PPY suggesting novel roles for these proteins in early development. OxJournal of assisted reproduction and genetics323-332https://www.ebi.ac.uk/biostudies/studies/S-EPMC10894797biostudies-literatureMicroarray evidence that 8-cell human embryos express some hormone family members including oxytocin.PMC10894797Kiessling AAHarris ALDinopoulou VLoutradis DDrakakis PfalseMicroarray evidence that 8-cell human embryos express some hormone family members including oxytocin.<h4>Objective</h4>This study is to discover hormone pathways active in early cleaving human embryos.<h4>Methods</h4>A list of 152 hormones and receptors were compiled to query the microarray database of mRNAs in 8-cell human embryos, two lines of human embryonic stem cells plus human fibroblasts before and after induced pluripotency.<h4>Results</h4>Over half of the 152 hormones and receptors were silent on the arrays of all cell types, and more were detected at high or moderate levels on the 8-cell arrays than on the pluripotent cell or fibroblast arrays. Eight hormone family genes were uniquely detected at least 22-fold higher on the 8-cell arrays than the stem cell arrays: AVPI1, CCK, CORT, FSTL4, GIP, GPHA2, OXT, and PPY suggesting novel roles for these proteins in early development. Ox2024-01-01T00:00:00Z2024 Feb2025-04-05T10:19:15.932Z2025-04-05T10:19:15.932ZS-EPMC108947973813387710.1007/s10815-023-03002-8