{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Corano Scheri K"],"funding":["NEI NIH HHS","NCI NIH HHS","Juvenile Diabetes Research Foundation","NIH"],"pagination":["e172062"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10896003"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(23)"],"pubmed_abstract":["The management of preretinal fibrovascular membranes, a devastating complication of advanced diabetic retinopathy (DR), remains challenging. We characterized the molecular profile of cell populations in these fibrovascular membranes to identify potentially new therapeutic targets. Preretinal fibrovascular membranes were surgically removed from patients and submitted for single-cell RNA-Seq (scRNA-Seq). Differential gene expression was implemented to define the transcriptomics profile of these cells and revealed the presence of endothelial, inflammatory, and stromal cells. Endothelial cell reclustering identified subclusters characterized by noncanonical transcriptomics profile and active angiogenesis. Deeper investigation of the inflammatory cells showed a subcluster of macrophages express"],"journal":["JCI insight"],"pubmed_title":["Single-cell transcriptomics analysis of proliferative diabetic retinopathy fibrovascular membranes reveals AEBP1 as fibrogenesis modulator."],"pmcid":["PMC10896003"],"funding_grant_id":["INO-2022-1112-A-N","R01 EY034486","NIH-R01-EY30121-A1","P30 CA060553","R01 EY030121","K08 EY030923","R01 EY032609"],"pubmed_authors":["Corano Scheri K","Tedeschi T","Lavine JA","Fawzi AA","Thomson BR"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomics analysis of proliferative diabetic retinopathy fibrovascular membranes reveals AEBP1 as fibrogenesis modulator.","description":"The management of preretinal fibrovascular membranes, a devastating complication of advanced diabetic retinopathy (DR), remains challenging. We characterized the molecular profile of cell populations in these fibrovascular membranes to identify potentially new therapeutic targets. Preretinal fibrovascular membranes were surgically removed from patients and submitted for single-cell RNA-Seq (scRNA-Seq). Differential gene expression was implemented to define the transcriptomics profile of these cells and revealed the presence of endothelial, inflammatory, and stromal cells. Endothelial cell reclustering identified subclusters characterized by noncanonical transcriptomics profile and active angiogenesis. Deeper investigation of the inflammatory cells showed a subcluster of macrophages express","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-05-29T09:42:11.026Z","creation":"2025-02-19T01:42:13.012Z"},"accession":"S-EPMC10896003","cross_references":{"pubmed":["37917183"],"doi":["10.1172/jci.insight.172062"]}}