biostudies-literatureUnknown16(1)Kim WJChong Kun Dang Bio Co. LtdThe gut microbiota plays a pivotal role in metabolic disorders, notably type 2 diabetes mellitus (T2DM). In this study, we investigated the synergistic potential of combining the effects of <i>Bifidobacterium longum</i> NBM7-1 (CKD1) with anti-diabetic medicines, Lobeglitazone^Ⓡ (LO), Sitagliptin^Ⓡ (SI), and Metformin^Ⓡ (Met), to alleviate hyperglycemia in a diabetic mouse model. CKD1 effectively mitigated insulin resistance, hepatic steatosis, and enhanced pancreatic β-cell function, as well as fortifying gut-tight junction integrity. In the same way, SI-CKD1 and Met- CKD1 synergistically improved insulin sensitivity and prevented hepatic steatosis, as evidenced by the modulation of key genes associated with insulin signaling, β-oxidation, gluconeogenesis, adGut microbes2319889https://www.ebi.ac.uk/biostudies/studies/S-EPMC10896159biostudies-literature<i>B. longum</i> CKD1 enhances the efficacy of anti-diabetic medicines through upregulation of IL- 22 response in type 2 diabetic mice.PMC10896159Kim WJLee CKim TYKim HHan GKim KHuh CSChoi YShin CHPark SYKil BJKim Mfalse<i>B. longum</i> CKD1 enhances the efficacy of anti-diabetic medicines through upregulation of IL- 22 response in type 2 diabetic mice.The gut microbiota plays a pivotal role in metabolic disorders, notably type 2 diabetes mellitus (T2DM). In this study, we investigated the synergistic potential of combining the effects of <i>Bifidobacterium longum</i> NBM7-1 (CKD1) with anti-diabetic medicines, Lobeglitazone^Ⓡ (LO), Sitagliptin^Ⓡ (SI), and Metformin^Ⓡ (Met), to alleviate hyperglycemia in a diabetic mouse model. CKD1 effectively mitigated insulin resistance, hepatic steatosis, and enhanced pancreatic β-cell function, as well as fortifying gut-tight junction integrity. In the same way, SI-CKD1 and Met- CKD1 synergistically improved insulin sensitivity and prevented hepatic steatosis, as evidenced by the modulation of key genes associated with insulin signaling, β-oxidation, gluconeogenesis, ad2024-01-01T00:00:00Z2024 Jan-Dec2026-05-09T03:10:34.409Z2025-05-29T21:39:01.639ZS-EPMC108961593839117810.1080/19490976.2024.2319889