biostudies-literatureMartino FANPCYT, ArgentinaNational Ministry of Health of ArgentinaPublic Health Ontario Laboratorye0294820https://www.ebi.ac.uk/biostudies/studies/S-EPMC10896549biostudies-literatureUnknown19(2)Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteria including carbapenem-resistant Enterobacterales. There are ten mcr genes described in different Gram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far the most frequent combination. In Argentina, mcr-1 gene was characterized only on plasmids belonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboring plasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolates carrying the mcr-1 gene were sequenced using next generation technologies. Three isolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226, ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1),PloS oneNew insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates.PMC10896549Préstamo BID-PICT-2021-I-A-0603Regular BudgetMartino FCorso AMelano RMenocal MAPetroni AFaccone DfalseNew insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates.Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteria including carbapenem-resistant Enterobacterales. There are ten mcr genes described in different Gram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far the most frequent combination. In Argentina, mcr-1 gene was characterized only on plasmids belonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboring plasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolates carrying the mcr-1 gene were sequenced using next generation technologies. Three isolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226, ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1),2024-01-01T00:00:00Z20242026-07-05T03:19:58.722Z2025-05-18T12:52:43.606ZS-EPMC108965493840807110.1371/journal.pone.0294820