biostudies-literatureUnknown18Rhodes CT<h4>Introduction</h4>Enhancer of zeste homolog 2 (Ezh2) is responsible for trimethylation of histone 3 at lysine 27 (H3K27me3), resulting in repression of gene expression. Here, we explore the role of Ezh2 in forebrain GABAergic interneuron development.<h4>Methods</h4>We removed <i>Ezh2</i> in the MGE by generating <i>Nkx2-1Cre;Ezh2</i> conditional knockout mice. We then characterized changes in MGE-derived interneuron fate and electrophysiological properties in juvenile mice, as well as alterations in gene expression, chromatin accessibility and histone modifications in the MGE.<h4>Results</h4>Loss of <i>Ezh2</i> increases somatostatin-expressing (SST+) and decreases parvalbumin-expressing (PV+) interneurons in the forebrain. We observe fewer MGE-derived interneurons in the first postnataFrontiers in cellular neuroscience1334244https://www.ebi.ac.uk/biostudies/studies/S-EPMC10899338biostudies-literatureLoss of Ezh2 in the medial ganglionic eminence alters interneuron fate, cell morphology and gene expression profiles.PMC10899338Sohn MLee SElisha LZhang YNaskar SRocha PPRhodes CTPetros TJStevenson PDale RKAsokumar DLee DRfalseLoss of Ezh2 in the medial ganglionic eminence alters interneuron fate, cell morphology and gene expression profiles.<h4>Introduction</h4>Enhancer of zeste homolog 2 (Ezh2) is responsible for trimethylation of histone 3 at lysine 27 (H3K27me3), resulting in repression of gene expression. Here, we explore the role of Ezh2 in forebrain GABAergic interneuron development.<h4>Methods</h4>We removed <i>Ezh2</i> in the MGE by generating <i>Nkx2-1Cre;Ezh2</i> conditional knockout mice. We then characterized changes in MGE-derived interneuron fate and electrophysiological properties in juvenile mice, as well as alterations in gene expression, chromatin accessibility and histone modifications in the MGE.<h4>Results</h4>Loss of <i>Ezh2</i> increases somatostatin-expressing (SST+) and decreases parvalbumin-expressing (PV+) interneurons in the forebrain. We observe fewer MGE-derived interneurons in the first postnata2024-01-01T00:00:00Z20242026-07-14T23:59:43.669Z2025-04-06T11:01:31.668ZS-EPMC108993383841965610.3389/fncel.2024.1334244