{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(2)"],"submitter":["Ma B"],"pubmed_abstract":["The stomata regulate CO<sub>2</sub> uptake and efficient water usage, thereby promoting drought stress tolerance. NAC proteins (NAM, ATAF1/2, and CUC2) participate in plant reactions following drought stress, but the molecular mechanisms underlying NAC-mediated regulation of stomatal movement are unclear. In this study, a novel NAC gene from <i>Reaumuria trigyna</i>, <i>RtNAC055</i>, was found to enhance drought tolerance via a stomatal closure pathway. It was regulated by RtMYC2 and integrated with jasmonic acid signaling and was predominantly expressed in stomata and root. The suppression of <i>RtNAC055</i> could improve jasmonic acid and H<sub>2</sub>O<sub>2</sub> production and increase the drought tolerance of transgenic <i>R. trigyna</i> callus. Ectopic expression of <i>RtNAC055</i> in the <i>Arabidopsis atnac055</i> mutant rescued its drought-sensitive phenotype by decreasing stomatal aperture. Under drought stress, overexpression of <i>RtNAC055</i> in poplar promoted ROS (H<sub>2</sub>O<sub>2</sub>) accumulation in stomata, which accelerated stomatal closure and maintained a high photosynthetic rate. Drought upregulated the expression of <i>PtRbohD/F</i>, <i>PtP5CS2</i>, and <i>PtDREB1</i>.<i>1</i>, as well as antioxidant enzyme activities in heterologous expression poplars. RtNAC055 promoted H<sub>2</sub>O<sub>2</sub> production in guard cells by directly binding to the promoter of <i>RtRbohE</i>, thus regulating stomatal closure. The stress-related genes <i>RtDREB1</i>.<i>1/P5CS1</i> were directly regulated by RtNAC055. These results indicate that RtNAC055 regulates stomatal closure by maintaining the balance between the antioxidant system and H<sub>2</sub>O<sub>2</sub> level, reducing the transpiration rate and water loss, and improving photosynthetic efficiency and drought resistance."],"journal":["Horticulture research"],"pagination":["uhae001"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10901477"],"repository":["biostudies-literature"],"pubmed_title":["RtNAC055 promotes drought tolerance via a stomatal closure pathway linked to methyl jasmonate/hydrogen peroxide signaling in <i>Reaumuria trigyna</i>."],"pmcid":["PMC10901477"],"pubmed_authors":["Yan L","Ma B","Bu X","Zhang J","Xie X","Zhang H","Zheng L","Guo S","Chen H","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"RtNAC055 promotes drought tolerance via a stomatal closure pathway linked to methyl jasmonate/hydrogen peroxide signaling in <i>Reaumuria trigyna</i>.","description":"The stomata regulate CO<sub>2</sub> uptake and efficient water usage, thereby promoting drought stress tolerance. NAC proteins (NAM, ATAF1/2, and CUC2) participate in plant reactions following drought stress, but the molecular mechanisms underlying NAC-mediated regulation of stomatal movement are unclear. In this study, a novel NAC gene from <i>Reaumuria trigyna</i>, <i>RtNAC055</i>, was found to enhance drought tolerance via a stomatal closure pathway. It was regulated by RtMYC2 and integrated with jasmonic acid signaling and was predominantly expressed in stomata and root. The suppression of <i>RtNAC055</i> could improve jasmonic acid and H<sub>2</sub>O<sub>2</sub> production and increase the drought tolerance of transgenic <i>R. trigyna</i> callus. Ectopic expression of <i>RtNAC055</i> in the <i>Arabidopsis atnac055</i> mutant rescued its drought-sensitive phenotype by decreasing stomatal aperture. Under drought stress, overexpression of <i>RtNAC055</i> in poplar promoted ROS (H<sub>2</sub>O<sub>2</sub>) accumulation in stomata, which accelerated stomatal closure and maintained a high photosynthetic rate. Drought upregulated the expression of <i>PtRbohD/F</i>, <i>PtP5CS2</i>, and <i>PtDREB1</i>.<i>1</i>, as well as antioxidant enzyme activities in heterologous expression poplars. RtNAC055 promoted H<sub>2</sub>O<sub>2</sub> production in guard cells by directly binding to the promoter of <i>RtRbohE</i>, thus regulating stomatal closure. The stress-related genes <i>RtDREB1</i>.<i>1/P5CS1</i> were directly regulated by RtNAC055. These results indicate that RtNAC055 regulates stomatal closure by maintaining the balance between the antioxidant system and H<sub>2</sub>O<sub>2</sub> level, reducing the transpiration rate and water loss, and improving photosynthetic efficiency and drought resistance.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-22T06:34:44.882Z","creation":"2025-04-05T21:50:40.983Z"},"accession":"S-EPMC10901477","cross_references":{"pubmed":["38419969"],"doi":["10.1093/hr/uhae001"]}}