biostudies-literaturePeng TJMinistry of Science and Technology, TaiwanTaipei City Hospital, TaiwanTri·Service General Hospital and the National Defense Medical CenterMinistry of Education in Taiwan2249-2261https://www.ebi.ac.uk/biostudies/studies/S-EPMC10903253biostudies-literatureUnknown248(23)Chemokines critically orchestrate the tumorigenesis, metastasis, and stemness features of cancer cells that lead to poor outcomes. High plasma levels of transforming growth factor-β1 (TGFβ1) correlate with poor prognostic features in advanced lung cancer patients, thus suggesting the importance of TGFβ1 in the lung tumor microenvironment. However, the role of chemokines in TGFβ1-induced tumor stemness features remains unclear. Here, we clarify the previously undocumented role of CXCL1 in TGFβ1-induced lung cancer stemness features. CXCL1 and its receptor CXCR2 were significantly upregulated in TGFβ1-induced lung cancer stem cells (CSCs). CXCL1 silencing (shCXCL1) suppressed stemness gene expression, tumorsphere formation, colony formation, drug resistance, and <i>in vivo</i> tumorigenicityExperimental biology and medicine (Maywood, N.J.)TGFβ1 induces CXCL1 to promote stemness features in lung cancer.PMC10903253111W31210109-2320-B-010-038110-2320-B-A49A-514-MY3110CRC-T210Sun GHPeng TJWu YCSun KHTang SJfalseTGFβ1 induces CXCL1 to promote stemness features in lung cancer.Chemokines critically orchestrate the tumorigenesis, metastasis, and stemness features of cancer cells that lead to poor outcomes. High plasma levels of transforming growth factor-β1 (TGFβ1) correlate with poor prognostic features in advanced lung cancer patients, thus suggesting the importance of TGFβ1 in the lung tumor microenvironment. However, the role of chemokines in TGFβ1-induced tumor stemness features remains unclear. Here, we clarify the previously undocumented role of CXCL1 in TGFβ1-induced lung cancer stemness features. CXCL1 and its receptor CXCR2 were significantly upregulated in TGFβ1-induced lung cancer stem cells (CSCs). CXCL1 silencing (shCXCL1) suppressed stemness gene expression, tumorsphere formation, colony formation, drug resistance, and <i>in vivo</i> tumorigenicity2023-01-01T00:00:00Z2023 Dec2025-04-04T20:07:48.649Z2025-04-04T20:07:48.649ZS-EPMC109032533815880810.1177/15353702231220662