{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pan J"],"funding":["Zhejiang Medical Science and Technology Projects","National Natural Science Foundation of China"],"pagination":["e13555"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10905343"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(3)"],"pubmed_abstract":["The liver is the most tolerogenic of transplanted organs. However, the mechanisms underlying liver transplant tolerance are not well understood. The comparison between liver transplantation tolerance and heart/kidney transplantation rejection will deepen our understanding of tolerance and rejection in solid organs. Here, we built a mouse model of liver, heart and kidney allograft and performed single-cell RNA sequencing of 66,393 cells to describe the cell composition and immune cell interactions at the early stage of tolerance or rejection. We also performed bulk RNA-seq of mouse liver allografts from Day 7 to Day 60 post-transplantation to map the dynamic transcriptional variation in spontaneous tolerance. The transcriptome of lymphocytes and myeloid cells were characterized and compared in three types of organ allografts. Cell-cell interaction networks reveal the coordinated function of Kupffer cells, macrophages and their associated metabolic processes, including insulin receptor signalling and oxidative phosphorylation in tolerance induction. Cd11b+ dendritic cells (DCs) in liver allografts were found to inhibit cytotoxic T cells by secreting anti-inflammatory cytokines such as Il10. In summary, we profiled single-cell transcriptome analysis of mouse solid organ allografts. We characterized the immune microenvironment of mouse organ allografts in the acute rejection state (heart, kidney) and tolerance state (liver)."],"journal":["Cell proliferation"],"pubmed_title":["Dissecting the immune discrepancies in mouse liver allograft tolerance and heart/kidney allograft rejection."],"pmcid":["PMC10905343"],"funding_grant_id":["81800658","2019330585","2019330597"],"pubmed_authors":["Li H","Wu J","Li J","Xiao Y","Chen H","Meng X","Mao J","Pan J","Yu C","Guo G","Ye F","Wang Y","Wu Y","Fei L"],"additional_accession":[]},"is_claimable":false,"name":"Dissecting the immune discrepancies in mouse liver allograft tolerance and heart/kidney allograft rejection.","description":"The liver is the most tolerogenic of transplanted organs. However, the mechanisms underlying liver transplant tolerance are not well understood. The comparison between liver transplantation tolerance and heart/kidney transplantation rejection will deepen our understanding of tolerance and rejection in solid organs. Here, we built a mouse model of liver, heart and kidney allograft and performed single-cell RNA sequencing of 66,393 cells to describe the cell composition and immune cell interactions at the early stage of tolerance or rejection. We also performed bulk RNA-seq of mouse liver allografts from Day 7 to Day 60 post-transplantation to map the dynamic transcriptional variation in spontaneous tolerance. The transcriptome of lymphocytes and myeloid cells were characterized and compared in three types of organ allografts. Cell-cell interaction networks reveal the coordinated function of Kupffer cells, macrophages and their associated metabolic processes, including insulin receptor signalling and oxidative phosphorylation in tolerance induction. Cd11b+ dendritic cells (DCs) in liver allografts were found to inhibit cytotoxic T cells by secreting anti-inflammatory cytokines such as Il10. In summary, we profiled single-cell transcriptome analysis of mouse solid organ allografts. We characterized the immune microenvironment of mouse organ allografts in the acute rejection state (heart, kidney) and tolerance state (liver).","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T20:40:51.114Z","creation":"2025-04-04T20:40:51.114Z"},"accession":"S-EPMC10905343","cross_references":{"pubmed":["37748771"],"doi":["10.1111/cpr.13555"]}}