{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(2)"],"submitter":["Xiong Q"],"pubmed_abstract":["Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully cultured in a customized microfluidic device to assess their response to different chemotherapeutic agents. The in vitro drug sensitivity results were also compared to patient-derived xenograft (PDX) models and clinical responses of patients. As a result, bladder cancer spheroids faithfully recapitulate the histopathological and genetic features of their corresponding parental tumors. Furthermore, the in vitro drug sensitivity outcomes of spheroids (<i>n</i> = 8) demonstrated a high level of correlation with the PDX (<i>n</i> = 2) and clinical response in patients (<i>n</i> = 2). Our study highlights the potential of combining bladder cancer spheroids and microfluidic devices as an efficient and accurate platform for personalized selection of chemotherapeutic agents."],"journal":["Bioengineering & translational medicine"],"pagination":["e10624"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10905551"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response."],"pmcid":["PMC10905551"],"pubmed_authors":["Zeng S","Fan W","Lin T","Xiong Q","Xu C","Gao H","Liu T","Zhang Z","Wei Q","Ge Y","Ying Y","Wang Z","Yu X"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response.","description":"Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully cultured in a customized microfluidic device to assess their response to different chemotherapeutic agents. The in vitro drug sensitivity results were also compared to patient-derived xenograft (PDX) models and clinical responses of patients. As a result, bladder cancer spheroids faithfully recapitulate the histopathological and genetic features of their corresponding parental tumors. Furthermore, the in vitro drug sensitivity outcomes of spheroids (<i>n</i> = 8) demonstrated a high level of correlation with the PDX (<i>n</i> = 2) and clinical response in patients (<i>n</i> = 2). Our study highlights the potential of combining bladder cancer spheroids and microfluidic devices as an efficient and accurate platform for personalized selection of chemotherapeutic agents.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-18T17:15:52.793Z","creation":"2025-04-07T04:46:46.556Z"},"accession":"S-EPMC10905551","cross_references":{"pubmed":["38435820"],"doi":["10.1002/btm2.10624"]}}