{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee SJ"],"funding":["NRF"],"pagination":["e26800"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10906407"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(5)"],"pubmed_abstract":["Epidermal Growth Factor (EGF), a protein pivotal in cell proliferation and survival, has recently shown promise in alleviating inflammation. This study investigates EGF's impact on M1 macrophages, exploring its potential for anti-inflammatory and anti-vasculogenic interactions with corneal endothelial cells (CECs). Polarized M1 macrophages treated with EGF exhibited a suppression of gene expressions related to inflammatory and vasculogenic signals. The anti-inflammatory effects of EGF were observed in co-culture systems with human CECs (HCECs), showcasing its ability to alter macrophage phenotypes. Exosomes derived from EGF-treated M1 macrophages demonstrated enriched proteomic profiles related to immune system regulation and inflammation inhibition. When applied as eye drops in murine corneas, EGF-conditioned M1 macrophage-derived exosomes effectively reduced inflammation and increased M2-related <i>ARG1</i> expression. This study highlights EGF's potential in mitigating inflammation in M1 macrophages and its delivery through exosomes to cultured HCECs and murine corneas, suggesting a novel therapeutic avenue for ocular surface anti-inflammatory treatments."],"journal":["Heliyon"],"pubmed_title":["EGF-conditioned M1 macrophages Convey reduced inflammation into corneal endothelial cells through exosomes."],"pmcid":["PMC10906407"],"funding_grant_id":["RS-2023-00209498","RS-2023-00219421"],"pubmed_authors":["Koh A","Lee SJ","Lee SH","Kim KW"],"additional_accession":[]},"is_claimable":false,"name":"EGF-conditioned M1 macrophages Convey reduced inflammation into corneal endothelial cells through exosomes.","description":"Epidermal Growth Factor (EGF), a protein pivotal in cell proliferation and survival, has recently shown promise in alleviating inflammation. This study investigates EGF's impact on M1 macrophages, exploring its potential for anti-inflammatory and anti-vasculogenic interactions with corneal endothelial cells (CECs). Polarized M1 macrophages treated with EGF exhibited a suppression of gene expressions related to inflammatory and vasculogenic signals. The anti-inflammatory effects of EGF were observed in co-culture systems with human CECs (HCECs), showcasing its ability to alter macrophage phenotypes. Exosomes derived from EGF-treated M1 macrophages demonstrated enriched proteomic profiles related to immune system regulation and inflammation inhibition. When applied as eye drops in murine corneas, EGF-conditioned M1 macrophage-derived exosomes effectively reduced inflammation and increased M2-related <i>ARG1</i> expression. This study highlights EGF's potential in mitigating inflammation in M1 macrophages and its delivery through exosomes to cultured HCECs and murine corneas, suggesting a novel therapeutic avenue for ocular surface anti-inflammatory treatments.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-08T06:10:54.508Z","creation":"2025-04-07T04:44:22.11Z"},"accession":"S-EPMC10906407","cross_references":{"pubmed":["38434401"],"doi":["10.1016/j.heliyon.2024.e26800"]}}