biostudies-literatureManandhar PHHS | NIH | National Cancer InstituteNIAID NIH HHSHHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious DiseasesNCI NIH HHS466-474https://www.ebi.ac.uk/biostudies/studies/S-EPMC10906969biostudies-literatureUnknown212(3)Tim-3 is a transmembrane protein that is best known for being highly expressed on terminally exhausted CD8+ T cells associated with chronic infection and tumors, although its expression is not limited to those settings. Tim-3 is also expressed by CD8+ T cells during acute infection and by multiple other immune cell types, including CD4+ Th1 and regulatory T cells, dendritic cells, and mast cells. In this study, we investigated the role of Tim-3 signaling on CD8+ T cell memory using a Tim-3 conditional knockout mouse model and mice lacking the signaling portion of the Tim-3 cytoplasmic domain. Together, our results indicate that Tim-3 has at most a modest effect on the formation and function of CD8+ memory T cells.Journal of immunology (Baltimore, Md. : 1950)Tim-3 Is Not Required for Establishment of CD8+ T Cell Memory to Lymphocytic Choriomeningitis Virus.PMC10906969T32 CA082084R01 AI138504R01 CA206517R03 AI131049Kane LPSzymczak-Workman ALManandhar PfalseTim-3 Is Not Required for Establishment of CD8+ T Cell Memory to Lymphocytic Choriomeningitis Virus.Tim-3 is a transmembrane protein that is best known for being highly expressed on terminally exhausted CD8+ T cells associated with chronic infection and tumors, although its expression is not limited to those settings. Tim-3 is also expressed by CD8+ T cells during acute infection and by multiple other immune cell types, including CD4+ Th1 and regulatory T cells, dendritic cells, and mast cells. In this study, we investigated the role of Tim-3 signaling on CD8+ T cell memory using a Tim-3 conditional knockout mouse model and mice lacking the signaling portion of the Tim-3 cytoplasmic domain. Together, our results indicate that Tim-3 has at most a modest effect on the formation and function of CD8+ memory T cells.2024-01-01T00:00:00Z2024 Feb2025-04-05T10:18:54.128Z2025-04-05T10:18:54.128ZS-EPMC109069693810841710.4049/jimmunol.2300401