biostudies-literatureThirion APoliomyelitis Research FoundationNorth-West UniversityNational Research Foundation33https://www.ebi.ac.uk/biostudies/studies/S-EPMC10907482biostudies-literatureUnknown20(2)<h4>Introduction</h4> Because cerebrospinal fluid (CSF) samples are difficult to obtain for paediatric HIV, few studies have attempted to profile neurometabolic dysregulation.<h4>Aim and objective</h4>The aim of this exploratory study was to profile the neurometabolic state of CSF from a South African paediatric cohort using GCxGC-TOF/MS. The study included 54 paediatric cases (< 12 years), 42 HIV-negative controls and 12 HIV-positive individuals.<h4>Results</h4>The results revealed distinct metabolic alterations in the HIV-infected cohort. In the PLS-DA model, 18 metabolites significantly discriminated between HIV-infected and control groups. In addition, fold-change analysis, Mann-Whitney U tests, and effect size measurements verified these findings. Notably, lactose, myo-inositol, and gMetabolomics : Official journal of the Metabolomic SocietyAn exploratory investigation of the CSF metabolic profile of HIV in a South African paediatric cohort using GCxGC-TOF/MS.PMC1090748223-96TTK2203165223/84Solomons RWilliams MELoots DTThirion AMason SfalseAn exploratory investigation of the CSF metabolic profile of HIV in a South African paediatric cohort using GCxGC-TOF/MS.<h4>Introduction</h4> Because cerebrospinal fluid (CSF) samples are difficult to obtain for paediatric HIV, few studies have attempted to profile neurometabolic dysregulation.<h4>Aim and objective</h4>The aim of this exploratory study was to profile the neurometabolic state of CSF from a South African paediatric cohort using GCxGC-TOF/MS. The study included 54 paediatric cases (< 12 years), 42 HIV-negative controls and 12 HIV-positive individuals.<h4>Results</h4>The results revealed distinct metabolic alterations in the HIV-infected cohort. In the PLS-DA model, 18 metabolites significantly discriminated between HIV-infected and control groups. In addition, fold-change analysis, Mann-Whitney U tests, and effect size measurements verified these findings. Notably, lactose, myo-inositol, and g2024-01-01T00:00:00Z2024 Mar2025-04-06T23:34:56.541Z2024-10-19T06:33:45.091ZS-EPMC109074823842714210.1007/s11306-024-02098-y