biostudies-literatureLiu YNatural Science Foundation of Hebei ProvinceThe Major Program of National Natural Science Foundation of ChinaThe Key Program of National Natural Science Foundation of China68https://www.ebi.ac.uk/biostudies/studies/S-EPMC10908211biostudies-literatureUnknown23(1)<h4>Background</h4>Stress is implicated in various pathological conditions leading to liver injury. Existing evidence suggests that excessive stress can induce mitochondrial damage in hepatocytes, yet the underlying mechanism remains unclear. Ceramide synthase 6 (CerS6)-derived C16:0 ceramide is recognised as a lipotoxic substance capable of causing mitochondrial damage. However, the role of CerS6 in stress has received insufficient attention. This study aimed to explore the involvement of CerS6 in stress-induced hepatic damage and its associated mechanisms.<h4>Methods</h4>The rat restraint stress model and a corticosterone (CORT)-induced hepatocyte stress model were employed for in vivo and in vitro experimental analyses, respectively. Changes in mitochondrial damage and ceramide metaboliLipids in health and diseaseThe effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury.PMC109082118213005582293651H2023206051Zhang GLiu YLi YMa CCong BDong YSun QWang CZhang XShi WSun ZWang LfalseThe effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury.<h4>Background</h4>Stress is implicated in various pathological conditions leading to liver injury. Existing evidence suggests that excessive stress can induce mitochondrial damage in hepatocytes, yet the underlying mechanism remains unclear. Ceramide synthase 6 (CerS6)-derived C16:0 ceramide is recognised as a lipotoxic substance capable of causing mitochondrial damage. However, the role of CerS6 in stress has received insufficient attention. This study aimed to explore the involvement of CerS6 in stress-induced hepatic damage and its associated mechanisms.<h4>Methods</h4>The rat restraint stress model and a corticosterone (CORT)-induced hepatocyte stress model were employed for in vivo and in vitro experimental analyses, respectively. Changes in mitochondrial damage and ceramide metaboli2024-01-01T00:00:00Z2024 Mar2025-04-26T22:38:43.155Z2025-04-06T17:15:28.67ZS-EPMC109082113843164510.1186/s12944-024-02019-x