{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Voogd L"],"funding":["Horizon 2020 Marie Skłodowska-Curie Actions","NIAID NIH HHS","Dutch Research Council (NWO)","National Institute of Health","Bill and Melinda Gates Foundation"],"pagination":["109233"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10909886"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(3)"],"pubmed_abstract":["HLA-E molecules can present self- and pathogen-derived peptides to both natural killer (NK) cells and T cells. T cells that recognize HLA-E peptides via their T cell receptor (TCR) are termed donor-unrestricted T cells due to restricted allelic variation of HLA-E. The composition and repertoire of HLA-E TCRs is not known so far. We performed TCR sequencing on CD8<sup>+</sup> T cells from 21 individuals recognizing HLA-E tetramers (TMs) folded with two <i>Mtb</i>-HLA-E-restricted peptides. We sorted HLA-E <i>Mtb</i> TM<sup>+</sup> and TM<sup>-</sup> CD8<sup>+</sup> T cells directly <i>ex vivo</i> and performed bulk RNA-sequencing and single-cell TCR sequencing. The identified TCR repertoire was diverse and showed no conservation between and within individuals. TCRs selected from our single-"],"journal":["iScience"],"pubmed_title":["<i>Mtb</i> HLA-E-tetramer-sorted CD8<sup>+</sup> T cells have a diverse TCR repertoire."],"pmcid":["PMC10909886"],"funding_grant_id":["R01 AI141315","U19 AI057229","R21 AI127133","13259"],"pubmed_authors":["Ottenhoff THM","Franken KLMC","van Meijgaarden KE","Drittij AMHF","Hagedoorn RS","Ruibal P","Heemskerk MHM","Voogd L","Joosten SA","Leitner JA","Unen VV","Steinberger P","Davis MM","Scriba TJ","Dingenouts CKE"],"additional_accession":[]},"is_claimable":false,"name":"<i>Mtb</i> HLA-E-tetramer-sorted CD8<sup>+</sup> T cells have a diverse TCR repertoire.","description":"HLA-E molecules can present self- and pathogen-derived peptides to both natural killer (NK) cells and T cells. T cells that recognize HLA-E peptides via their T cell receptor (TCR) are termed donor-unrestricted T cells due to restricted allelic variation of HLA-E. The composition and repertoire of HLA-E TCRs is not known so far. We performed TCR sequencing on CD8<sup>+</sup> T cells from 21 individuals recognizing HLA-E tetramers (TMs) folded with two <i>Mtb</i>-HLA-E-restricted peptides. We sorted HLA-E <i>Mtb</i> TM<sup>+</sup> and TM<sup>-</sup> CD8<sup>+</sup> T cells directly <i>ex vivo</i> and performed bulk RNA-sequencing and single-cell TCR sequencing. The identified TCR repertoire was diverse and showed no conservation between and within individuals. TCRs selected from our single-","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-07-15T00:18:15.071Z","creation":"2026-06-28T03:06:20.409Z"},"accession":"S-EPMC10909886","cross_references":{"pubmed":["38439958"],"doi":["10.1016/j.isci.2024.109233"]}}