{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27(3)"],"submitter":["Tan J"],"funding":["Temasek Life Sciences Laboratory Ltd"],"pubmed_abstract":["ApoE regulates neurogenesis, although how it influences genetic programs remains elusive. Cortical neurons induced from isogenic control and <i>ApoE-/-</i> human neural stem cells (NSCs) recapitulated key transcriptomic signatures of <i>in vivo</i> counterparts identified from single-cell human midbrain. Surprisingly, ApoE expression in NSC and neural progenitor cells (NPCs) is not required for differentiation. Instead, ApoE prevents the over-proliferation of non-neuronal cells during extended neuronal culture when it is not expressed. Elevated <i>miR-199a-5p</i> level in <i>ApoE-/-</i> cells lowers the EZH1 protein and the repressive H3K27me3 mark, a phenotype rescued by <i>miR-199a-5p</i> steric inhibitor. Reduced H3K27me3 at genes linked to extracellular matrix organization and angiogen"],"journal":["iScience"],"pagination":["109231"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10909902"],"repository":["biostudies-literature"],"pubmed_title":["ApoE maintains neuronal integrity via microRNA and H3K27me3-mediated repression."],"pmcid":["PMC10909902"],"pubmed_authors":["Rao VK","Ong CT","Tan J","Ngian ZK","Zeng X","Wang JW","Chong SY","Tan YY"],"additional_accession":[]},"is_claimable":false,"name":"ApoE maintains neuronal integrity via microRNA and H3K27me3-mediated repression.","description":"ApoE regulates neurogenesis, although how it influences genetic programs remains elusive. Cortical neurons induced from isogenic control and <i>ApoE-/-</i> human neural stem cells (NSCs) recapitulated key transcriptomic signatures of <i>in vivo</i> counterparts identified from single-cell human midbrain. Surprisingly, ApoE expression in NSC and neural progenitor cells (NPCs) is not required for differentiation. Instead, ApoE prevents the over-proliferation of non-neuronal cells during extended neuronal culture when it is not expressed. Elevated <i>miR-199a-5p</i> level in <i>ApoE-/-</i> cells lowers the EZH1 protein and the repressive H3K27me3 mark, a phenotype rescued by <i>miR-199a-5p</i> steric inhibitor. Reduced H3K27me3 at genes linked to extracellular matrix organization and angiogen","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-16T04:50:03.344Z","creation":"2025-04-19T22:01:35.661Z"},"accession":"S-EPMC10909902","cross_references":{"pubmed":["38439966"],"doi":["10.1016/j.isci.2024.109231"]}}