biostudies-literatureUnknown15Tang N<h4>Background</h4>Acute lung injury (ALI)/severe acute respiratory distress syndrome (ARDS) is a serious clinical syndrome characterized by a high mortality rate. The pathophysiological mechanisms underlying ALI/ARDS remain incompletely understood. Considering the crucial role of immune infiltration and macrophage polarization in the pathogenesis of ALI/ARDS, this study aims to identify key genes associated with both ALI/ARDS and M1 macrophage polarization, employing a combination of bioinformatics and experimental approaches. The findings could potentially reveal novel biomarkers for the diagnosis and management of ALI/ARDS.<h4>Methods</h4>Gene expression profiles relevant to ALI were retrieved from the GEO database to identify co-upregulated differentially expressed genes (DEGs). GO andFrontiers in immunology1344805https://www.ebi.ac.uk/biostudies/studies/S-EPMC10909908biostudies-literature<i>CD274</i> (PD-L1) negatively regulates M1 macrophage polarization in ALI/ARDS.PMC10909908Yang GLiu ZYang YXie YWang QLi CTang NHuang JAfalse<i>CD274</i> (PD-L1) negatively regulates M1 macrophage polarization in ALI/ARDS.<h4>Background</h4>Acute lung injury (ALI)/severe acute respiratory distress syndrome (ARDS) is a serious clinical syndrome characterized by a high mortality rate. The pathophysiological mechanisms underlying ALI/ARDS remain incompletely understood. Considering the crucial role of immune infiltration and macrophage polarization in the pathogenesis of ALI/ARDS, this study aims to identify key genes associated with both ALI/ARDS and M1 macrophage polarization, employing a combination of bioinformatics and experimental approaches. The findings could potentially reveal novel biomarkers for the diagnosis and management of ALI/ARDS.<h4>Methods</h4>Gene expression profiles relevant to ALI were retrieved from the GEO database to identify co-upregulated differentially expressed genes (DEGs). GO and2024-01-01T00:00:00Z20242026-06-16T04:50:50.456Z2025-04-19T22:02:45.069ZS-EPMC109099083844072210.3389/fimmu.2024.1344805