biostudies-literatureThongsa-Ad UMinistry of Science and Technology of ThailandMahidol UniversityRamathibodi FoundationFaculty of Science, Mahidol UniversityNational Research Council of Thailand60https://www.ebi.ac.uk/biostudies/studies/S-EPMC10910864biostudies-literatureUnknown15(1)<h4>Background</h4>The diarylheptanoid ASPP 049 has improved the quality of adult hematopoietic stem cell (HSC) expansion ex vivo through long-term reconstitution in animal models. However, its effect on hematopoietic regeneration from human induced pluripotent stem cells (hiPSCs) is unknown.<h4>Method</h4>We utilized a defined cocktail of cytokines without serum or feeder followed by the supplementation of ASPP 049 to produce hematopoietic stem/progenitor cells (HSPCs). Flow cytometry and trypan blue exclusion analysis were used to identify nonadherent and adherent cells. Nonadherent cells were harvested to investigate the effect of ASPP 049 on multipotency using LTC-IC and CFU assays. Subsequently, the mechanism of action was explored through transcriptomic profiles, which were validatedStem cell research & therapyImproving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative.PMC10910864the Central Instrument Facility (CIF)the Science Achievement Scholarship of ThailandMU’s Strategic Research Fund: fiscal year 2023N41A660154MU's Strategic Research Fund: fiscal year 2023Wongpan ATong-Ngam PChukhan ATubsuwan AWongniam SHongeng SUppakara KPabuprappap WBhukhai KAnurathapan UWongkummool WThongsa-Ad UChaiwijit PKulkeaw KSingpant PChaiyakitpattana GSuksamrarn AfalseImproving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative.<h4>Background</h4>The diarylheptanoid ASPP 049 has improved the quality of adult hematopoietic stem cell (HSC) expansion ex vivo through long-term reconstitution in animal models. However, its effect on hematopoietic regeneration from human induced pluripotent stem cells (hiPSCs) is unknown.<h4>Method</h4>We utilized a defined cocktail of cytokines without serum or feeder followed by the supplementation of ASPP 049 to produce hematopoietic stem/progenitor cells (HSPCs). Flow cytometry and trypan blue exclusion analysis were used to identify nonadherent and adherent cells. Nonadherent cells were harvested to investigate the effect of ASPP 049 on multipotency using LTC-IC and CFU assays. Subsequently, the mechanism of action was explored through transcriptomic profiles, which were validated2024-01-01T00:00:00Z2024 Mar2026-06-16T04:50:51.866Z2025-04-06T17:15:23.208ZS-EPMC109108643843321710.1186/s13287-024-03686-4