{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sharifi KA"],"funding":["NCI NIH HHS","NINDS NIH HHS"],"pagination":["1353142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10915230"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18"],"pubmed_abstract":["The Wnt pathway plays critical roles in neurogenesis. The expression of <i>Axin2</i> is induced by Wnt/β-catenin signaling, making this gene a reliable indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the <i>Axin2-CreERT2</i> allele to follow the fate of <i>Axin2</i>+ lineage in the adult hippocampal formation. We found <i>Axin2</i> expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with the induction of <i>Axin2</i> fate mapping by tamoxifen, we marked the dividing cells with 5-ethynyl-2'-deoxyuridine (EdU). Tamoxifen induction led to a significant increase in labeled dentate gyrus granule cells three months later. However, none of these neurons showed any EdU signal. Conversely, six months after the pulse-chase labeling with tamoxifen/EdU, we identified granule neurons that were positive for both EdU and tdTomato lineage tracer in each animal. Our data indicates that <i>Axin2</i> is expressed at multiple stages of adult granule neuron differentiation. Furthermore, these findings suggest that the integration process of adult-born neurons from specific cell lineages may require more time than previously thought."],"journal":["Frontiers in neuroscience"],"pubmed_title":["Exploring the dynamics of adult <i>Axin2</i> cell lineage integration into dentate gyrus granule neurons."],"pmcid":["PMC10915230"],"funding_grant_id":["R01 CA279134","R21 NS116431"],"pubmed_authors":["Soldozy S","DeWitt MR","Farzad F","Kalani MYS","Sheehan J","Tvrdik P","Price RJ","Sharifi KA"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the dynamics of adult <i>Axin2</i> cell lineage integration into dentate gyrus granule neurons.","description":"The Wnt pathway plays critical roles in neurogenesis. The expression of <i>Axin2</i> is induced by Wnt/β-catenin signaling, making this gene a reliable indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the <i>Axin2-CreERT2</i> allele to follow the fate of <i>Axin2</i>+ lineage in the adult hippocampal formation. We found <i>Axin2</i> expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with the induction of <i>Axin2</i> fate mapping by tamoxifen, we marked the dividing cells with 5-ethynyl-2'-deoxyuridine (EdU). Tamoxifen induction led to a significant increase in labeled dentate gyrus granule cells three months later. However, none of these neurons showed any EdU signal. Conversely, six months after the pulse-chase labeling with tamoxifen/EdU, we identified granule neurons that were positive for both EdU and tdTomato lineage tracer in each animal. Our data indicates that <i>Axin2</i> is expressed at multiple stages of adult granule neuron differentiation. Furthermore, these findings suggest that the integration process of adult-born neurons from specific cell lineages may require more time than previously thought.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-19T09:59:50.837Z","creation":"2025-04-19T09:59:50.837Z"},"accession":"S-EPMC10915230","cross_references":{"pubmed":["38449734"],"doi":["10.3389/fnins.2024.1353142"]}}