biostudies-literatureSharifi KANCI NIH HHSNINDS NIH HHS1353142https://www.ebi.ac.uk/biostudies/studies/S-EPMC10915230biostudies-literatureUnknown18The Wnt pathway plays critical roles in neurogenesis. The expression of <i>Axin2</i> is induced by Wnt/β-catenin signaling, making this gene a reliable indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the <i>Axin2-CreERT2</i> allele to follow the fate of <i>Axin2</i>+ lineage in the adult hippocampal formation. We found <i>Axin2</i> expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with the induction of <i>Axin2</i> fate mapping by tamoxifen, we marked the dividing cells with 5-ethynyl-2'-deoxyuridine (EdU). Tamoxifen induction led to a significant increase in labeled dentate gyrus granule cells three months later. However, none of these neurons showed any EdU signal. Conversely, six months after the pulse-chase labeling with tamoxifen/EdU, we identified granule neurons that were positive for both EdU and tdTomato lineage tracer in each animal. Our data indicates that <i>Axin2</i> is expressed at multiple stages of adult granule neuron differentiation. Furthermore, these findings suggest that the integration process of adult-born neurons from specific cell lineages may require more time than previously thought.Frontiers in neuroscienceExploring the dynamics of adult <i>Axin2</i> cell lineage integration into dentate gyrus granule neurons.PMC10915230R01 CA279134R21 NS116431Soldozy SDeWitt MRFarzad FKalani MYSSheehan JTvrdik PPrice RJSharifi KAfalseExploring the dynamics of adult <i>Axin2</i> cell lineage integration into dentate gyrus granule neurons.The Wnt pathway plays critical roles in neurogenesis. The expression of <i>Axin2</i> is induced by Wnt/β-catenin signaling, making this gene a reliable indicator of canonical Wnt activity. We employed pulse-chase genetic lineage tracing with the <i>Axin2-CreERT2</i> allele to follow the fate of <i>Axin2</i>+ lineage in the adult hippocampal formation. We found <i>Axin2</i> expressed in astrocytes, neurons and endothelial cells, as well as in the choroid plexus epithelia. Simultaneously with the induction of <i>Axin2</i> fate mapping by tamoxifen, we marked the dividing cells with 5-ethynyl-2'-deoxyuridine (EdU). Tamoxifen induction led to a significant increase in labeled dentate gyrus granule cells three months later. However, none of these neurons showed any EdU signal. Conversely, six months after the pulse-chase labeling with tamoxifen/EdU, we identified granule neurons that were positive for both EdU and tdTomato lineage tracer in each animal. Our data indicates that <i>Axin2</i> is expressed at multiple stages of adult granule neuron differentiation. Furthermore, these findings suggest that the integration process of adult-born neurons from specific cell lineages may require more time than previously thought.2024-01-01T00:00:00Z20242025-04-19T09:59:50.837Z2025-04-19T09:59:50.837ZS-EPMC109152303844973410.3389/fnins.2024.1353142