{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["de Hoyos-Vega JM"],"funding":["NIDDK NIH HHS","Mayo Clinic","NCI NIH HHS","National Institutes of Health"],"pagination":["115896"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10916504"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["248"],"pubmed_abstract":["A common challenge in microfluidic cell cultures has to do with analysis of cell function without replacing a significant fraction of the culture volume and disturbing local concentration gradients of signals. To address this challenge, we developed a microfluidic cell culture device with an integrated bioanalysis unit to enable on-chip analysis of picoliter volumes of cell-conditioned media. The culture module consisted of an array of 140 microwells with a diameter of 300 m which were made low-binding to promote organization of cells into 3D spheroids. The bioanalysis module contained a droplet generator unit, 15 micromechanical valves and reservoirs loaded with reagents. Each 0.8 nL droplet contained an aliquot of conditioned media mixed with assay reagents. The use of microvalves allowe"],"journal":["Biosensors & bioelectronics"],"pubmed_title":["Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit."],"pmcid":["PMC10916504"],"funding_grant_id":["DK134661","R21 CA236612","DK084567","R01 DK111378","P30 DK084567","R21 CA126716","R01 DK107255","CA236612","R01 DK134661","DK107255"],"pubmed_authors":["Garcia-Cordero JL","Stybayeva G","Matveyenko A","de Hoyos-Vega JM","Gonzalez-Suarez AM","Revzin A","Malhi H","Cedillo-Alcantar DF"],"additional_accession":[]},"is_claimable":false,"name":"Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit.","description":"A common challenge in microfluidic cell cultures has to do with analysis of cell function without replacing a significant fraction of the culture volume and disturbing local concentration gradients of signals. To address this challenge, we developed a microfluidic cell culture device with an integrated bioanalysis unit to enable on-chip analysis of picoliter volumes of cell-conditioned media. The culture module consisted of an array of 140 microwells with a diameter of 300 m which were made low-binding to promote organization of cells into 3D spheroids. The bioanalysis module contained a droplet generator unit, 15 micromechanical valves and reservoirs loaded with reagents. Each 0.8 nL droplet contained an aliquot of conditioned media mixed with assay reagents. The use of microvalves allowe","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T09:07:37.596Z","creation":"2025-04-04T09:07:37.596Z"},"accession":"S-EPMC10916504","cross_references":{"pubmed":["38176252"],"doi":["10.1016/j.bios.2023.115896"]}}