{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35(3)"],"submitter":["van der Beek J"],"pubmed_abstract":["The multisubunit HOPS tethering complex is a well-established regulator of lysosome fusion with late endosomes and autophagosomes. However, the role of the HOPS complex in other stages of endo-lysosomal trafficking is not well understood. To address this, we made HeLa cells knocked out for the HOPS-specific subunits Vps39 or Vps41, or the HOPS-CORVET-core subunits Vps18 or Vps11. In all four knockout cells, we found that endocytosed cargos were trapped in enlarged endosomes that clustered in the perinuclear area. By correlative light-electron microscopy, these endosomes showed a complex ultrastructure and hybrid molecular composition, displaying markers for early (Rab5, PtdIns3P, EEA1) as well as late (Rab7, CD63, LAMP1) endosomes. These \"HOPS bodies\" were not acidified, contained enzymati"],"journal":["Molecular biology of the cell"],"pagination":["ar40"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10916860"],"repository":["biostudies-literature"],"pubmed_title":["Loss of the HOPS complex disrupts early-to-late endosome transition, impairs endosomal recycling and induces accumulation of amphisomes."],"pmcid":["PMC10916860"],"pubmed_authors":["de Heus C","Sanza P","Liv N","van der Beek J","Klumperman J"],"additional_accession":[]},"is_claimable":false,"name":"Loss of the HOPS complex disrupts early-to-late endosome transition, impairs endosomal recycling and induces accumulation of amphisomes.","description":"The multisubunit HOPS tethering complex is a well-established regulator of lysosome fusion with late endosomes and autophagosomes. However, the role of the HOPS complex in other stages of endo-lysosomal trafficking is not well understood. To address this, we made HeLa cells knocked out for the HOPS-specific subunits Vps39 or Vps41, or the HOPS-CORVET-core subunits Vps18 or Vps11. In all four knockout cells, we found that endocytosed cargos were trapped in enlarged endosomes that clustered in the perinuclear area. By correlative light-electron microscopy, these endosomes showed a complex ultrastructure and hybrid molecular composition, displaying markers for early (Rab5, PtdIns3P, EEA1) as well as late (Rab7, CD63, LAMP1) endosomes. These \"HOPS bodies\" were not acidified, contained enzymati","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T12:58:22.744Z","creation":"2025-04-04T12:58:22.744Z"},"accession":"S-EPMC10916860","cross_references":{"pubmed":["38198575"],"doi":["10.1091/mbc.E23-08-0328"]}}