{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Miller SG"],"funding":["NCI NIH HHS","NIH HHS","NIGMS NIH HHS"],"pagination":["ar31"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10916866"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(3)"],"pubmed_abstract":["The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of WRAMP structures is melanoma cell adhesion molecule (MCAM) which dynamically polarizes to the cell rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using MCAM as bait identifies the ERM family proteins, moesin, ezrin, and radixin, as WRAMP structure components. We also present a novel image analysis pipeline, Protein Polarity by Percentile (\"3P\"), which classifies protein polarization using machine learning and facilitates quantitative analysis. Using 3P, we find that depletion of moesin, and to a lesser extent ezrin, decreases the proportion of cells with polarized MCAM. "],"journal":["Molecular biology of the cell"],"pubmed_title":["Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma."],"pmcid":["PMC10916866"],"funding_grant_id":["P30 CA046934","R01 GM127986","R01 CA107098","R35 GM136392","S10 OD025267","T32 GM008759","S10 OD021601"],"pubmed_authors":["Hoh M","Tay JW","Ahn NG","Miller SG","Ebmeier CC"],"additional_accession":[]},"is_claimable":false,"name":"Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma.","description":"The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of WRAMP structures is melanoma cell adhesion molecule (MCAM) which dynamically polarizes to the cell rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using MCAM as bait identifies the ERM family proteins, moesin, ezrin, and radixin, as WRAMP structure components. We also present a novel image analysis pipeline, Protein Polarity by Percentile (\"3P\"), which classifies protein polarization using machine learning and facilitates quantitative analysis. Using 3P, we find that depletion of moesin, and to a lesser extent ezrin, decreases the proportion of cells with polarized MCAM. ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-07-15T16:09:17.931Z","creation":"2025-04-04T09:33:51.152Z"},"accession":"S-EPMC10916866","cross_references":{"pubmed":["38117590"],"doi":["10.1091/mbc.E23-06-0255"]}}