biostudies-literatureUnknown43(3)Acheampong IUniversitätsklinikum Düsseldorf. Anstalt öffentlichen RechtsImpaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis-specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38⁺ T-cell proportions specific for M. tuberculosis protein (PPD_Mtb), yet total proportions of PPD_Mtb-specific T-cells were comparable. Notably, both activated (CD38⁺) and total IFN-γ⁺ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology611-616https://www.ebi.ac.uk/biostudies/studies/S-EPMC10917863biostudies-literatureDifferences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients.PMC10917863Abass MKMayatepek EYeboah AAcheampong IJacobsen MLamptey MDebrah LBKumbel FPhillips ROFrimpong MOsei-Yeboah FArthur JFDebrah AOwusu DOSeyfarth JAniagyei WLaing EFMinadzi DAdankwah EVivekanandan MMGawusu AfalseDifferences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients.Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis-specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38⁺ T-cell proportions specific for M. tuberculosis protein (PPD_Mtb), yet total proportions of PPD_Mtb-specific T-cells were comparable. Notably, both activated (CD38⁺) and total IFN-γ⁺ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.2024-01-01T00:00:00Z2024 Mar2026-06-12T10:08:28.814Z2026-06-12T03:12:19.116ZS-EPMC109178633816798710.1007/s10096-023-04741-3