biostudies-literatureUnknown15Wenzlau JMThere is accumulating evidence that pathogenic T cells in T1D recognize epitopes formed by post-translational modifications of β-cell antigens, including hybrid insulin peptides (HIPs). The ligands for several CD4 T-cell clones derived from the NOD mouse are HIPs composed of a fragment of proinsulin joined to peptides from endogenous β-cell granule proteins. The diabetogenic T-cell clone BDC-6.9 reacts to a fragment of C-peptide fused to a cleavage product of pro-islet amyloid polypeptide (6.9HIP). In this study, we used a monoclonal antibody (MAb) to the 6.9HIP to determine when and where HIP antigens are present in NOD islets during disease progression and with which immune cells they associate. Immunogold labeling of the 6.9HIP MAb and organelle-specific markers for electron microscopy Frontiers in immunology1348131https://www.ebi.ac.uk/biostudies/studies/S-EPMC10917911biostudies-literatureMapping of a hybrid insulin peptide in the inflamed islet β-cells from NOD mice.PMC10917911Hohenstein ADiLisio JEHaskins KPeterson OJWenzlau JMWan XVomund ANfalseMapping of a hybrid insulin peptide in the inflamed islet β-cells from NOD mice.There is accumulating evidence that pathogenic T cells in T1D recognize epitopes formed by post-translational modifications of β-cell antigens, including hybrid insulin peptides (HIPs). The ligands for several CD4 T-cell clones derived from the NOD mouse are HIPs composed of a fragment of proinsulin joined to peptides from endogenous β-cell granule proteins. The diabetogenic T-cell clone BDC-6.9 reacts to a fragment of C-peptide fused to a cleavage product of pro-islet amyloid polypeptide (6.9HIP). In this study, we used a monoclonal antibody (MAb) to the 6.9HIP to determine when and where HIP antigens are present in NOD islets during disease progression and with which immune cells they associate. Immunogold labeling of the 6.9HIP MAb and organelle-specific markers for electron microscopy 2024-01-01T00:00:00Z20242025-04-04T12:34:46.471Z2025-04-04T12:34:46.471ZS-EPMC109179113845505510.3389/fimmu.2024.1348131