{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nie X"],"funding":["Tianjin Science and Technology Project","Tianjin Health Research Project","National Key Research and Development Program","National Nature Science Foundation of China"],"pagination":["rbae012"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10918636"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["3D printing of high-strength natural polymer biodegradable hydrogel scaffolds simultaneously resembling the biomechanics of corneal tissue and facilitating tissue regeneration remains a huge challenge due to the inherent brittleness of natural polymer hydrogels and the demanding requirements of printing. Herein, concentrated aqueous solutions of gelatin and carbohydrazide-modified alginate (Gel/Alg-CDH) are blended to form a natural polymer hydrogel ink, where the hydrazides in Alg-CDH are found to form strong hydrogen bonds with the gelatin. The hydrogen-bonding-strengthened Gel/Alg-CDH hydrogel demonstrates an appropriate thickened viscosity and shear thinning for extrusion printing. The strong hydrogen bonds contribute to remarkably increased mechanical properties of Gel/Alg-CDH hydrogel with a maximum elongation of over 400%. In addition, sequentially Ca<sup>2+</sup>-physical crosslinking and then moderately chemical crosslinking significantly enhance the mechanical properties of Gel/Alg-CDH hydrogels that ultimately exhibit an intriguing J-shaped stress-strain curve (tensile strength of 1.068 MPa and the toughness of 677.6 kJ/m<sup>2</sup>). The dually crosslinked Gel-Alg-CDH-Ca<sup>2+</sup>-EDC hydrogels demonstrate a high transparency, physiological swelling stability and rapid enzymatic degradability, as well as suturability. The growth factor and drug-loaded biomimetic bilayer hydrogel scaffold are customized via a multi-nozzle printing system. This bioactive bilayer hydrogel scaffold considerably promotes regeneration of corneal epithelium and stroma and inhibits cornea scarring in rabbit cornea keratoplasty."],"journal":["Regenerative biomaterials"],"pubmed_title":["3D printing sequentially strengthening high-strength natural polymer hydrogel bilayer scaffold for cornea regeneration."],"pmcid":["PMC10918636"],"funding_grant_id":["TJWJ2022ZD009","22JCZDJC00280","52233008","2018YFA0703100"],"pubmed_authors":["Han Q","Wu B","Yang R","Wu T","Hui J","Sun Y","Nie X","Tang Y","Xu Z","Zhao X","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"3D printing sequentially strengthening high-strength natural polymer hydrogel bilayer scaffold for cornea regeneration.","description":"3D printing of high-strength natural polymer biodegradable hydrogel scaffolds simultaneously resembling the biomechanics of corneal tissue and facilitating tissue regeneration remains a huge challenge due to the inherent brittleness of natural polymer hydrogels and the demanding requirements of printing. Herein, concentrated aqueous solutions of gelatin and carbohydrazide-modified alginate (Gel/Alg-CDH) are blended to form a natural polymer hydrogel ink, where the hydrazides in Alg-CDH are found to form strong hydrogen bonds with the gelatin. The hydrogen-bonding-strengthened Gel/Alg-CDH hydrogel demonstrates an appropriate thickened viscosity and shear thinning for extrusion printing. The strong hydrogen bonds contribute to remarkably increased mechanical properties of Gel/Alg-CDH hydrogel with a maximum elongation of over 400%. In addition, sequentially Ca<sup>2+</sup>-physical crosslinking and then moderately chemical crosslinking significantly enhance the mechanical properties of Gel/Alg-CDH hydrogels that ultimately exhibit an intriguing J-shaped stress-strain curve (tensile strength of 1.068 MPa and the toughness of 677.6 kJ/m<sup>2</sup>). The dually crosslinked Gel-Alg-CDH-Ca<sup>2+</sup>-EDC hydrogels demonstrate a high transparency, physiological swelling stability and rapid enzymatic degradability, as well as suturability. The growth factor and drug-loaded biomimetic bilayer hydrogel scaffold are customized via a multi-nozzle printing system. This bioactive bilayer hydrogel scaffold considerably promotes regeneration of corneal epithelium and stroma and inhibits cornea scarring in rabbit cornea keratoplasty.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-04T12:34:31.675Z","creation":"2025-04-04T12:34:31.675Z"},"accession":"S-EPMC10918636","cross_references":{"pubmed":["38454966"],"doi":["10.1093/rb/rbae012"]}}