biostudies-literatureLathe RNIA NIH HHS5209-5231https://www.ebi.ac.uk/biostudies/studies/S-EPMC10918877biostudies-literatureUnknown19(11)Microbial infections of the brain can lead to dementia, and for many decades microbial infections have been implicated in Alzheimer's disease (AD) pathology. However, a causal role for infection in AD remains contentious, and the lack of standardized detection methodologies has led to inconsistent detection/identification of microbes in AD brains. There is a need for a consensus methodology; the Alzheimer's Pathobiome Initiative aims to perform comparative molecular analyses of microbes in post mortem brains versus cerebrospinal fluid, blood, olfactory neuroepithelium, oral/nasopharyngeal tissue, bronchoalveolar, urinary, and gut/stool samples. Diverse extraction methodologies, polymerase chain reaction and sequencing techniques, and bioinformatic tools will be evaluated, in addition to diAlzheimer's & dementia : the journal of the Alzheimer's AssociationEstablishment of a consensus protocol to explore the brain pathobiome in patients with mild cognitive impairment and Alzheimer's disease: Research outline and call for collaboration.PMC10918877P30 AG066530P30 AG062429Doty RLSchultek NMEkberg JNara PLSacco GSt John JHammond CBell NDIntracell Research Group Consortium CollaboratorsTanzi REMoein STBalin BJAuber LACorry DBRissman RAWhittum-Hudson JHahn DLLathe RItzhaki RHaas JGEhrlich GDPerry GAlbensi BCMcLaughlin GImbimbo BPKonig AEimer WAHudson APBreitschwerdt EBLinz NNelson MLfalseEstablishment of a consensus protocol to explore the brain pathobiome in patients with mild cognitive impairment and Alzheimer's disease: Research outline and call for collaboration.Microbial infections of the brain can lead to dementia, and for many decades microbial infections have been implicated in Alzheimer's disease (AD) pathology. However, a causal role for infection in AD remains contentious, and the lack of standardized detection methodologies has led to inconsistent detection/identification of microbes in AD brains. There is a need for a consensus methodology; the Alzheimer's Pathobiome Initiative aims to perform comparative molecular analyses of microbes in post mortem brains versus cerebrospinal fluid, blood, olfactory neuroepithelium, oral/nasopharyngeal tissue, bronchoalveolar, urinary, and gut/stool samples. Diverse extraction methodologies, polymerase chain reaction and sequencing techniques, and bioinformatic tools will be evaluated, in addition to di2023-01-01T00:00:00Z2023 Nov2026-06-01T23:48:07.145Z2024-11-09T06:22:16.652ZS-EPMC109188773728326910.1002/alz.13076