{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen H"],"funding":["Research Project of Jinan Microecological Biomedicine Shandong Laboratory","Shandong Provincial Laboratory Project","the Fundamental Research Funds for the Central Universities"],"pagination":["24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10918965"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(1)"],"pubmed_abstract":["<h4>Background</h4>Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics.<h4>Methods</h4>Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis.<h4>Results</h4>K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The bla<sub>IMP-4</sub> gene was located on the In809-like integrative element (Intl1-bla<sub>IMP-4</sub>-aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae.<h4>Conclusions</h4>This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub>. It is highly likely that the strain acquired this plasmid through horizontal transfer. The bla<sub>IMP-4</sub> array (Intl1-bla<sub>IMP-4</sub>-aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying bla<sub>IMP-4</sub>, and the In809 integrative element."],"journal":["Annals of clinical microbiology and antimicrobials"],"pubmed_title":["Coexistence of bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain."],"pmcid":["PMC10918965"],"funding_grant_id":["SYS202202","2022ZFJH003","JNL-2022001A"],"pubmed_authors":["Shen J","Zheng B","Liu R","Chen H","Xu H","Li L"],"additional_accession":[]},"is_claimable":false,"name":"Coexistence of bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain.","description":"<h4>Background</h4>Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics.<h4>Methods</h4>Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis.<h4>Results</h4>K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub> genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The bla<sub>IMP-4</sub> gene was located on the In809-like integrative element (Intl1-bla<sub>IMP-4</sub>-aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae.<h4>Conclusions</h4>This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring bla<sub>IMP-4</sub> and bla<sub>SFO-1</sub>. It is highly likely that the strain acquired this plasmid through horizontal transfer. The bla<sub>IMP-4</sub> array (Intl1-bla<sub>IMP-4</sub>-aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying bla<sub>IMP-4</sub>, and the In809 integrative element.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-22T03:11:34.634Z","creation":"2025-04-04T12:35:26.785Z"},"accession":"S-EPMC10918965","cross_references":{"pubmed":["38448920"],"doi":["10.1186/s12941-024-00680-9"]}}