biostudies-literatureUnknown12Su RRThe intestinal bacteria of longhorn beetles would be ideal targets for pest control and lignocellulosic resources by destroying or exploiting their cellulose-degrading function. This article aims to investigate the diversity and community structure of intestinal bacteria the oligophagous longhorn beetle <i>Glenea cantor</i>. Additionally, it seeks to identify the presence of lignocellulose-degrading bacteria in the gut, and explore their role in consuming host kapok trees <i>Bombax malabaricum</i>. In this study, the bacterial community from <i>G. cantor</i> was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) targeting the V3 and V4 regions. A total of 563,201 valid sequences and 814 OTUs were obtained. The dominant phyla were Proteobacteria, and the dominant genera were <i>Acinetobacter</i> and Lactococcus. The analysis of microbial diversity revealed a high bacterial diversity in the samples, with the gut bacteria playing a crucial role in the physiological activities of the host, particularly, 9 genera of intestinal bacteria with cellulose degradation function were found, highlighting their vital role in cellulose degradation. Five strains of cellulose-degrading bacteria, belonging to the genus <i>Pseudomonas,</i> were obtained from the intestinal tract of <i>G. cantor</i> larvae using traditional isolation and culture techniques as well as 16S rDNA sequencing. Among these strains, A4 exhibited a cellulase activity of 94.42 ± 0.42 U/mL, while A5 displayed the highest filter paper enzyme activity of 127.46 ± 3.54 U/mL. These results offered valuable insights into potential targets for pest control through internal attack digestion and cellulose-degrading bacteria in longhorn beetles.Frontiers in bioengineering and biotechnology1340168https://www.ebi.ac.uk/biostudies/studies/S-EPMC10919226biostudies-literatureCharacterization of bacterial diversity and screening of cellulose-degrading bacteria in the gut system of <i>Glenea cantor</i> (Fabricius) larvae.PMC10919226Lu WSu RRZheng XLLuo YXPan BQWang XYfalseCharacterization of bacterial diversity and screening of cellulose-degrading bacteria in the gut system of <i>Glenea cantor</i> (Fabricius) larvae.The intestinal bacteria of longhorn beetles would be ideal targets for pest control and lignocellulosic resources by destroying or exploiting their cellulose-degrading function. This article aims to investigate the diversity and community structure of intestinal bacteria the oligophagous longhorn beetle <i>Glenea cantor</i>. Additionally, it seeks to identify the presence of lignocellulose-degrading bacteria in the gut, and explore their role in consuming host kapok trees <i>Bombax malabaricum</i>. In this study, the bacterial community from <i>G. cantor</i> was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) targeting the V3 and V4 regions. A total of 563,201 valid sequences and 814 OTUs were obtained. The dominant phyla were Proteobacteria, and the dominant genera were <i>Acinetobacter</i> and Lactococcus. The analysis of microbial diversity revealed a high bacterial diversity in the samples, with the gut bacteria playing a crucial role in the physiological activities of the host, particularly, 9 genera of intestinal bacteria with cellulose degradation function were found, highlighting their vital role in cellulose degradation. Five strains of cellulose-degrading bacteria, belonging to the genus <i>Pseudomonas,</i> were obtained from the intestinal tract of <i>G. cantor</i> larvae using traditional isolation and culture techniques as well as 16S rDNA sequencing. Among these strains, A4 exhibited a cellulase activity of 94.42 ± 0.42 U/mL, while A5 displayed the highest filter paper enzyme activity of 127.46 ± 3.54 U/mL. These results offered valuable insights into potential targets for pest control through internal attack digestion and cellulose-degrading bacteria in longhorn beetles.2024-01-01T00:00:00Z20242026-06-21T03:11:18.645Z2025-04-04T12:35:19.101ZS-EPMC109192263845600310.3389/fbioe.2024.1340168